可用于尿酸检测的钯纳米粒子的制备及初步应用  

The preparation and application of palladium nanoparticles using in uric acid detection preliminaryly

作　　者：邹瑟音 周念 黄文秀 凌连生[2] 曹东林[1] 雷达[1] ZOU Seyin;ZHOU Nian;HUANG Wenxiu;LING Liansheng;CAO Donglin;LEI Da(Department of Clinical Laboratory Medicine,Guangdong Second Provincial General Hospital,Guangzhou,Guangdong 518037,China;College of Chemical Engineering,Sun Yat-sen University,Guangzhou,Guangdong 510275,China)

机构地区：[1]广东省第二人民医院检验医学部,广东广州518037 [2]中山大学化学与工程学院,广东广州510275

出　　处：《国际检验医学杂志》2020年第22期2744-2747,2751,共5页International Journal of Laboratory Medicine

基　　金：广东省第二人民医院青年科研项目(YQ2017-008);广东省中医药局课题面上项目(20202004)。

摘　　要：目的制备具有过氧化物酶活性的钯纳米粒子,并初步探讨其应用于尿酸检测的可行性。方法通过简单的一步法化学反应合成具有氢键超分子结构的钯纳米粒子复合物(MCA-Pd NPs),应用比色分析方法检测其过氧化物酶活性。再设计一系列的试验寻找到纳米粒子应用于尿酸检测体系的最佳反应时间、温度、试剂用量等,并通过制作尿酸标准曲线、检测临床血清标本来探索其临床应用的可行性。结果成功合成性质稳定、具有强过氧化物酶活性的MCA-Pd NPs,并能取代辣根过氧化物酶应用到尿酸检测体系中,检测体系的最佳试剂用量为100μL MCA-Pd NPs(0.105 mg/mL)和50μL ABTS2-(90 mmol/L),最佳反应温度为37℃,最佳反应时间为20 min。纳米粒子比色法检测的5份临床血清标本与日立7600生化分析仪检测结果相一致,与临床诊断相符合。结论钯纳米粒子制作过程简单、性质稳定、类过氧化物酶活性强,并基于该纳米粒子和尿酸酶建立了一种可初步应用于临床血清标本尿酸检测的比色分析方法。Objective To prepare palladium nanoparticles with peroxidase activity and use in uric acid detection preliminaryly.Methods Synthesis of the palladium nanoparticles with hydrogen bonding supramolecular structure by a simple one-step reaction and the peroxidase activity was detected using the colorimetric analysis.To find the optimum reaction time,temperature,dosage of regents of uric acid detection system involving palladium nanoparticles through a series of experiments.To explore the feasibility of its clinical application by standard curve production and clinical serum samples test.Results Successfully synthesised the palladium nanoparticles with stability and high peroxidase activity and could be applied in the uric acid detection system instead of horseradish peroxidase.The best dosage,optimum reaction temperature and time of this system was 100μL MCA-Pd NPs(0.105 mg/mL)and 50μL ABTS2-(90 mmol/L),37℃,20 min respectively.The results of 5 clinical serum samples of palladium nanoparticles colorimetric analysis were consistent with those of Hitachi 7600 biochemical analyzer,being in keeping with the clinical diagnosis.Conclusion The palladium nanoparticles has the characteristics of making simply,stable nature and strong peroxidase activity,a colorimetric analysis is successfully established basing on palladium particles and uric acid enzyme to test uric acid.

关 键 词：钯纳米粒子 过氧化物酶 尿酸 比色分析 

分 类 号：R446.9[医药卫生—诊断学]