生物纳米FeS和磁性炭对Klebsiella sp.LZ6降解芘效果的影响  

Effects of bio-nano FeS and magnetic carbon on pyrene degradation by Klebsiella sp.LZ6

作　　者：张雪英 李莲 祝佩茹 林超霸 雍晓雨[2] 刘奋武[3] 周俊[2] ZHANG Xueying;LI Lian;ZHU Peiru;LIN Chaoba;YONG Xiaoyu;LIU Fenwu;ZHOU Jun(School of Environmental Science and Engineering, Nanjing Tech University, Nanjing 211800, China;College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800,China;College of Resources and Environment, Shanxi Agricultural University, Taigu 030801, China)

机构地区：[1]南京工业大学环境科学与工程学院,江苏南京211800 [2]南京工业大学生物与制药工程学院,江苏南京211800 [3]山西农业大学资源环境学院,山西太谷030801

出　　处：《南京工业大学学报（自然科学版）》2020年第6期804-813,共10页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家自然科学基金(21777069);宁夏回族自治区重点研发计划(2019BFH02008);山西省重点研发计划(201903D211013)。

摘　　要：芘是土壤、沉积物和水中的一种具有较强的致畸、致突变和致癌作用的有机污染物,能抑制微生物的生长,从而影响微生物降解芘的能力。本文通过摇瓶试验考察生物纳米FeS和磁性炭分别对Klebsiella sp.LZ6生长的影响,探究这两种材料分别作为生物载体协同Klebsiella sp.LZ6厌氧降解芘的可行性。结果表明:在分别添加生物纳米FeS和磁性炭后,Klebsiella sp.LZ6与材料粘附在一起,Klebsiella sp.LZ6细胞膜形态没有发生改变,且促进了细胞的生长,菌体浓度得到了提高;当芘的质量浓度为50 mg/L时,生物纳米FeS+Klebsiella sp.LZ6和磁性炭+Klebsiella sp.LZ6的两组反应体系芘的降解率分别为53.10%和48.24%,均高于Klebsiella sp.LZ6单独降解芘的降解率(28.06%),生物纳米FeS和磁性炭均能协同Klebsiella sp.LZ6提高芘的降解率。通过气相色谱质谱联用分析确定Klebsiella sp.LZ6降解芘的中间产物有4,5二氢芘、菲、1,1′(1亚丁烯基)双苯、氢化肉桂酸、2,6二羟基苯乙酮、1,2苯二甲酸二丙酯、2,5二羟基苯甲酸以及一些短链酸,加入生物纳米FeS或磁性炭的中间产物与对照组中有所不同,可能是添加的材料影响了相关酶的表达,导致代谢途径发生了变化。生物纳米FeS+Klebsiella sp.LZ6组代谢产物中多出2(3,4二羟基苯基)2羟基乙酸、苯甲酸、丁酸、丙酸和对苯酚等中间产物,而磁性炭+Klebsiella sp.LZ6组代谢产物中存在己烯雌酚和1,3二苯基1烯1醇。Pyrene is a organic pollutant in soil,sediment and water,which has the charactistic of teratogenic,mutagenic and carcinogenic.It can inhibit the growth of microorganisms and affect the efficiency of pyrene degradation.The effects of bio-nano FeS and magnetic carbon on the growth of Klebsiella sp.LZ6 were studied by shake flask experiment.The feasibility of using bio-nano FeS or magnetic carbon as bio-carriers to enhance the anaerobic degradation of pyrene by Klebsiella sp.LZ6 was investigated.Results showed that Klebsiella sp.LZ6 adhered to bio-nano FeS or magnetic carbon,the cell membrane morphology of Klebsiella sp.LZ6 did not change,the bacterial growth was enhanced,and the cell concentration was increased after adding bio-nano FeS or magnetic carbon.When the mass concentration of pyrene was 50 mg/L,the pyrene removal rates reached 53.10%in the bio-nano FeS+Klebsiella sp.LZ6 system and 48.24%in the magnetic carbon+Klebsiella sp.LZ6 system,whereas it was only 28.06%in the Klebsiella sp.LZ6 control system.The results suggested that adding bio-nano FeS or magnetic carbon could improve the efficiency of pyrene degradation.According to gas chromatography-mass spectrometer analysis,the intermediate metabolites of Klebsiella sp.LZ6 were identified,such as 4,5-dihydropyrene,phenanthrene,benzene,1,1′-(1-butenylidene)bis-,hydrocinnamicacid,1-(2,6-dihydroxyphenyl)ethan-one,1,2-benzenedicarboxylic acid,dipropylester,2-hydroxy-5-methylbenzoic acid and some short-chain acids.The expression of related enzymes may be affected,resulting in changes in metabolic pathways when bio-nano FeS or magnetic carbon was added.There may be 2-(3,4-dihydroxyphenyl)-2-hydroxyacetic acid,benzoic acid,butyric acid,propionic acid,p-cresol in the metabolites after adding bio-nano FeS,and there may be hexestrol and 1,3-diphenylpent-1-ene-1-ol in the presence of magnetic carbon.

关 键 词：生物纳米FeS 磁性炭 Klebsiella sp.LZ6 生物降解 芘 

分 类 号：X53[环境科学与工程—环境工程] Q93[生物学—微生物学]