肾衰方及其拆方对肾间质纤维化VEGF、VEGFR2、a-SMA的影响  

Effects of Shenshuai Formula and Its Decomposed Formulae on VEGF,VEGFR2 and a-SMA in Renal Interstitial Fibrosis

作　　者：晏子友[1] 杨芸琪 沈金峰 胡芳 罗富里 黄伟[2] 皮持衡[1] YAN Ziyou;YANG Yunqi;SHEN Jinfeng;HU Fang;LUO Fuli;HUANG Wei;PI Chiheng(Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,Jiangxi,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 430000,Jiangxi,China;Wenling First People’s Hospital,Wenling 317500,Zhejiang,China)

机构地区：[1]江西中医药大学附属医院,江西南昌330006 [2]江西中医药大学,江西南昌430000 [3]温岭市第一人民医院,浙江温岭317500

出　　处：《中华中医药学刊》2020年第10期10-15,I0012,I0013,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81660769);江西省自然科学基金(20171BAB205079)。

摘　　要：目的探讨肾衰方及其拆方对单侧输尿管梗阻(UUO)大鼠肾间质纤维化(RIF)及VEGF、VEGFR2、a-SMA的影响。方法采用单侧输尿管梗阻建立RIF大鼠模型。将108只无特定病原体(SPF)级健康成年SD雌性大鼠随机分为假手术组、模型组、肾衰方组、祛邪方组、补虚方组、贝那普利组,每组18只。UUO术后1 d起连续灌胃给药3周,肾衰方组、补虚方组、祛邪方组大鼠分别按19.5、9.5、11.0 g/(kg·d)进行灌胃,贝那普利组给予1.05 mg/(kg·d),假手术组和模型组每日给予等量生理盐水灌胃。造模后各组分别在第7、14、21天各处死6只大鼠,检测其血清肌酐(Scr)、尿素氮(BUN),同时计算左肾肾脏脏器指数;采用苏木精-伊红染色和马松染色进行肾脏组织病理检测,采用Western Blot检测VEGF、VEGFR2、a-SMA的表达,实时荧光定量RT-PCR检测VEGF、VEGFR2、a-SMAmRNA的表达。结果(1)与假手术组比较,同时期模型组、肾衰方组、祛邪方组、补虚方组、贝那普利组血清BUN、Scr、左肾肾脏脏器指数均升高(P<0.05)。与模型组相比,同时期肾衰方组、祛邪方组、补虚方组、贝那普利组血BUN、Scr、肾脏脏器指数均降低(P<0.05),与其他治疗组相比,肾衰方组在降低BUN、Scr、肾脏脏器指数更明显(P<0.05)。(2)肾脏病理改变:与假手术组比较,同时期模型组、肾衰方组、祛邪方组、补虚方组、贝那普利组肾小管间质损伤指数和胶原阳性面积均增加(P<0.05)。与UUO模型组比较,同时期肾衰方组、祛邪方组、补虚方组、贝那普利组肾小管间质损伤指数和胶原阳性面积均降低(P<0.05);与其他治疗组相比,肾衰方组在减轻肾小管间质损伤指数和胶原阳性面积更明显(P<0.05)。(3)与假手术组比较,同时期模型组、肾衰方组、祛邪方组、补虚方组、贝那普利组VEGF、VEGFR2、a-SMA蛋白和VEGF、VEGFR2、a-SMAmRNA表达量均增加(P<0.05);与模型组相比,同时期肾衰方组、祛邪方组Objective To investigate the effects of Shenshuai Formula and its decomposed formulae on renal interstitial fibrosis(RIF),expressions of VEGF, VEGFR2 and a-SMA in UUO rats. Methods RIF rat model was established by unilateral ureteral obstruction(UUO). A total of 108 healthy adult SD female rats without specific pathogen(SPF) were randomly divided into sham operation group, model group, Shenshuai Formula group, Quxie Formula group, Buxu Formula group and benazepril group, 18 in each group. UUO was administered by intragastric administration for 3 weeks from the 1 st day after operation. The rats in the Shenshuai Formula group, Buxu Formula group and Quxie Formula group were given the corresponding formula with the dose of 19.5 g/(kg·d), 9.5 g/(kg·d) and 11.0 g/(kg·d) respectively. The benazepril group was given benazepril with a dose of 1.05 mg/(kg·d). The sham operation group and the model group were given the same amount of normal saline per day. After model establishment, 6 rats died on the 7 th, 14 th and 21 st day, and their serum levels of creatinine(Scr) and urea nitrogen(BUN) were detected. The left kidney organ index was also calculated. The kidney tissue was detected by HE staining and Masson staining. The expressions of VEGF, VEGFR2 and a-SMA were detected by Western blot. The expressions of VEGF, VEGFR2 and a-SMAmRNA were detected by real-time quantitative RT-PCR. Results(1)Compared with those of the sham operation group, the serum levels of BUN, Scr, and left kidney organ index were increased in the model group, Shenshuai Formula group, Quxie Formula group, Buxu Formula group and benazepril group(P<0.05). Compared with those of the model group, the levels of BUN and Scr and kidney organ index of the Shenshuai Formula group,Quxie Formula group, Buxu Formula group and benazepril group were decreased(P<0.05). Compared with those of the other treatment groups,the Shenshuai Formula group’s levels of BUN, Scr and kidney organ index were significantly lower(P<0.05).(2)Renal pathological changes: Compar

关 键 词：肾衰方 UUO大鼠 肾间质纤维化 a-SAM VEGF VEGFR2 

分 类 号：R285.5[医药卫生—中药学]