基于网络药理学苍耳散治疗鼻炎作用机制研究  

Mechanism of Cang’er Powder in Treatment of Rhinitis Based on Network Pharmacology

作　　者：胡聪 黎红维 吴琳静 熊印华 HU Cong;LI Hongwei;WU Linjing;XIONG Yinhua(School of Pharmacy,Jiangxi Science and Technology Normal University,Nanchang 330013,Jiangxi,China;Jiangxi Provincial Key Laboratory of Drug Design and Evaluation,Nanchang 330013,Jiangxi,China)

机构地区：[1]江西科技师范大学药学院,江西南昌330013 [2]江西省药物分子设计与评价重点实验室,江西南昌330013

出　　处：《中华中医药学刊》2020年第10期64-71,I0026,I0027,共10页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81660692);江西省教育厅科学技术研究项目(GJJ170683);江西省药物分子设计与评价重点实验室(20171BCD40015)。

摘　　要：目的运用网络药理学的方法探讨苍耳散治疗鼻炎的作用机制。方法利用中药系统药理数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)获得苍耳散中的活性化学成分及其相应靶点,利用OMIM、Gene Cards、DigSee 3个数据库获取鼻炎相关的疾病靶点,并将药物靶点与疾病靶点取交集得到苍耳散作用于鼻炎的预测靶点;使用Cytoscape 3.7.1软件来建立单味药、活性成分、作用靶点相互作用关系的网络图;借助String平台和Cytoscape 3.7.1来构建蛋白相互作用(PPI)网络;运用Systems dock Web Site对关键活性成分及关键靶点进行分子对接,验证网络分析结果;利用生物学信息注释数据库(DAVID)对苍耳散作用靶点进行基因本体(gene ontology,GO)功能富集分析以及基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。结果苍耳散中的活性成分有57个,作用于鼻炎的靶点有72个;关键活性成分主要包括木犀草素、柚皮素、谷甾醇等;关键靶点主要包括丝裂原活化蛋白激酶1(MAPK1)、丝裂原活化蛋白激酶3(MAPK3)、白细胞介素-6(IL-6)等;对接结果显示,预测的关键活性成分与关键靶点具有较好的结合活性;基因富集分析得到GO功能条目1393个(P<0.01),通路129条(P<0.01)。结论苍耳散对鼻炎的治疗作用体现了中药及其复方多成分、多靶点、多途径的特点,为进一步研究其治疗鼻炎的作用机制提供了新的线索。Objective To investigate the mechanism of Cang’er Powder for rhinitis treatment based on network pharmacology.Method The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)was used to obtain the active chemical compositions of Cang’er Powder and corresponding targets,and the related targets of rhinitis were provided by OMIM,Gene Cards and DigSee databases.Then,the predicted targets of Cang’er Powder acting on rhinitis were obtained by mapping the drug targets to the disease targets.Cytoscape 3.7.1 was used to construct the network of“single drug-active ingredient-action targets”,and the protein interaction network(PPI)was constructed by String platform and Cytoscape 3.7.1.The molecular docking of key active components and key targets was carried out by Systems dock Web Site,which was used to verify the results of network analysis.Gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the action targets of Cang’er Powder by biological information annotation dotabase(DAVID).Result There were 57 active compounds in Cang’er Powder,and 72 targets for the treatment of rhinitis.The key active compounds mainly included luteolin,naringenin and β-sitosterol,etc,and the key targets primarily included mitogen-activated protein kinase 1(MAPK1),mitogen-activated protein kinase 3(MAPK3)and interleukin-6(IL-6),etc.Docking results showed that the predicted key active components had good binding activity to the key targets.A total of 1393 GO functional entries(P<0.01)and 129 metabolic pathways(P<0.01)were yielded by gene enrichment analysis.Conclusion The therapeutic mechanism of Cang’er Powder reflects the characteristics of multi-component,multi-target and multi-pathway of traditional Chinese medicine,which provides new clues for further research on its mechanism in treating rhinitis.

关 键 词：网络药理学 苍耳散 鼻炎 靶点 通路 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]