2-羟丙基-β-环糊精对白藜芦醇的包合作用研究  

Study on the Inclusion Effect of 2-Hydroxypropyl-β-Cyclodextrin on Resveratrol

作　　者：王晴晴 王晓楠 余双双 李文 李昭瑩 蒋雄 邓乾华 苟文琼 唐佳丽 杨善彬[1,2] 李霄 李标 蒲道俊 WANG Qingqing;WANG Xiaonan;YU Shuangshuang;LI Wen;LI Zhaoying;JIANG Xiong;DENG Qianhua;GOU Wenqiong;TANG Jiali;YANG Shanbin;LI Xiao;LI Biao;PU Daojun(College of Chemistry,Chongqing Normal University,Chongqing 401331;Engineering Research Center for Biotechnology of Active Substances,Ministry of Education,Chongqing Normal University,Chongqing 401331;Chongqing Enterprise Key Laboratory of Sustained and Controlled Release Preparations of Southwest Pharmaceutical Co.,Ltd.,Chongqing 400038,China)

机构地区：[1]重庆师范大学化学学院 [2]重庆师范大学活性物质生物技术教育部工程研究中心,重庆401331 [3]西南药业股份有限公司缓释控制剂重庆市企业重点实验室,重庆400038

出　　处：《重庆师范大学学报（自然科学版）》2020年第5期109-114,共6页Journal of Chongqing Normal University:Natural Science

基　　金：重庆市高校创新团队项目(No.CXTDX201601018);活性物质生物技术教育部工程研究中心开放基金项目(No.20150408,No.AS201903);重庆师范大学成果转化基金(No.15XZH08);重庆师范大学博士启动项目(No.12XLB006);重庆市大学生创新创业训练计划项目(No.S201910637014,No.S202010637012);重庆市社会事业与民生保障科技创新专项项目(No.cstc2015shmszx80060)。

摘　　要：目的】探索用包合物增加白藜芦醇的水溶性。【方法】在单因素实验的基础上,将包合率作为考察指标,采用4因素3水平正交试验法,优化白藜芦醇/2-羟丙基-β-环糊精(2-HP-β-CD)包合物的制备条件。【结果】在投料比为1∶2,包合温度为40℃,包合时间为50min,搅拌速率为865r·min^-1,最终测得白藜芦醇/2-HP-β-CD包合物的包合率为78.78%,通过核磁共振、红外光谱、X-射线衍射等表征验证了白藜芦醇/2-HP-β-CD包合物的形成。【结论】经过包合的白藜芦醇在水中溶解度由0.03g·L^-1提高到27.85g·L^-1。[Purposes]To investigate the effect of inclusion on resveratrol for increasing its solubility in water.[Methods]On the basis of single-factor experiment,the inclusion rate was used as the inspection index,and the four-factor three-level orthogonal test method was used to optimize the preparation conditions of resveratrol/2-HP-β-cyclodextrin inclusion compound.[Findings]The optimal preparation conditions were as follows:feeding ratio 1∶2,inclusion temperature 40℃,inclusion time 50 min,stirring rate865 r·min^-1,and the inclusion rate of resveratrol/2-HP-β-cyclodextrin inclusion compound was 78.78%.The formation of inclusion compounds was characterized by nuclear magnetic resonance,infrared spectroscopy,and X-Ray diffraction.[Conclusions]After forming resveratrol/2-HP-β-cyclodextrin inclusion compound,the solubility of resveratrol in water was raised from 0.03 g·L^-1 to27.85 g·L^-1.

关 键 词：白藜芦醇 2-羟丙基-Β-环糊精 包合物 正交试验 

分 类 号：R284.1[医药卫生—中药学]