三种试剂检测新型冠状病毒肺炎康复者恢复期血浆抗体的结果分析  

Performance evaluation of 3 assays in detecting serum IgG and IgM antibody titre in recovered COVID-19 patients

作　　者：王锦[1] 李恒新 黄蕾[1] 徐华[1] 李凤琴[1] WANG Jin;LI Hengxin;HUANG Lei;XU Hua;LI Fengqin(Shaarui Blood Center,Xi'an 710000,China.)

机构地区：[1]陕西省血液中心西安市中心血站,陕西西安710000

出　　处：《中国输血杂志》2020年第8期750-752,共3页Chinese Journal of Blood Transfusion

基　　金：西安市卫健委面上培育项目(2020ms17)

摘　　要：目的评价胶体金法和化学发光法试剂检测新型冠状病毒肺炎康复者恢复期血浆抗体的检测性能。方法对22例恢复期血浆按照《血站技术操作规程(2019版)》进行质量检测和SARS-CoV-2核酸检测,并采用2种胶体金法和1种化学发光法试剂检测血浆中新冠抗体IgG/IgM水平。结果 22份标本的一般质量检测中,1例ALT复检不合格。2种胶体金法和化学发光法试剂在新冠抗体IgM检测中,阳性率分别为54.5%(12/22)、31.8%(7/22)和72.7%(16/22),2种胶体金法试剂检测结果存在差异(P<0.05),2种胶体金法试剂与化学发光法试剂检测结果差别无统计学意义(P>0.05);在新冠抗体IgG检测中,阳性率分别为68.2%(15/22),81.8%(18/22)和86.4%(19/22),2种胶体金法试剂检测结果存在差异(P<0.05),2种胶体金法与化学发光法试剂检测结果存在差异(P<0.05)。结论 3种型冠状病毒抗体检测试剂的检测能力有差异,需根据临床需要选择合适的试剂。Objective To evaluate the performance of 2 kits of colloidal gold assays and 1 kit of chemiluminescent assay in detecting serum IgG and IgM antibody titre in recovered COVID-19 patients. Methods The quality test and SARS-CoV-2 NAT of 22 convalescent plasma(CP) specimens were conducted according to Blood Station Technical Operation Regulation(Version2019). The IgG and IgM antibody titer in CP were detected by 2 kits of colloidal gold assays and 1 kit of chemiluminescence assay in parallel. Results One deferral in 22 samples was detected due to unqualified ALT results.3 kits of assays yielded equivalent incidences of positive IgM(P>0.05): 72.7%(16/22)in one chemiluminescence assay, and 54.5%(12/22)and 31.8%(7/22)in two colloidal gold assays, among which significant difference in positive IgM rate was noticed(P<0.05).The positive incidence of IgG antibody in three kits of assays was 68.2%(15/22), 81.8%(18/22) and 86.4%(19/22),respectively. Significant differences either among the 3 assays or between 2 colloidal gold assays were observed(P<0.05).Conclusion Considering the existing differences in performance, suitable reagent should be selected according to practical clinical needs.

关 键 词：新型冠状病毒 恢复期血浆 抗体检测 

分 类 号：R457.[医药卫生—治疗学]