武汉808人份新冠康复者血浆的制备特性  

Characteristics of convalescent plasma preparation specific to COVID-19,a study of 808 recovered COVID-19 patients in Wuhan

作　　者：刘川桥[1] 郑雪[1] 王炼红 秋凤华 薛炼[1] LIU Chuanqiao;ZHENG Xue;WANG Lianhong;QIU Fenghua;XUE Lian(Wuhan Blood Center Wuhan 430030,China.Corresponding)

机构地区：[1]武汉血液中心,湖北武汉430030

出　　处：《中国输血杂志》2020年第8期758-760,共3页Chinese Journal of Blood Transfusion

摘　　要：目的采集新型冠状病毒肺炎康复者恢复期血浆,进行血液成分制备用于临床新冠危重病患者治疗。方法采集新冠康复出院14 d以上且无发热并符合献血者健康标准的捐献者血浆进行血液成分制备,全封闭无菌分装、亚甲蓝病毒灭活、血浆速冻制备成新冠康复者血浆和病毒灭活新冠康复者血浆供应临床。结果 808人份康复者血浆中A型占39.1%、B型占26.2%、O型占25.4%、AB型占9.3%,检验合格603人份占74.6%,不合格205人份占25.4%(其中血清学IgG抗体<160不合格159人份,其他传染性指标不合格46人份);共制备分装捐献者血浆1 699袋,其中制备新冠康复者血浆133袋(100 mL规格132袋、200 mL规格1袋);制备病毒灭活新冠康复者血浆1 566袋(100 mL规格642袋、150 mL规格4袋、200 mL规格920袋)。结论规范新冠康复者血浆成分制备特性,完善安全有效的制备和防护措施,有效提高临床新冠康复者血浆的安全性和治疗效果。Objective To collect the plasma from some recovered COVID-19 patients to prepare therapeutic convalescent plasma(CP) for clinical treatment of critical ill COVID-19 patients. Methods After strict blood biosafety tests, plasma derived from recently recovered COVID-19 donors(discharged more than 14 days prior) with normal bady temperature were subjected to aseptic package and quick-freezing to prepare CP, and methylene blue virus inactivation as an addition to prepare virus inactivated CP.Results 808 recovered COVID-19 patients participated in PC donation, and 39.1%, 26.2%, 25.4% and 9.3% of them were blood type A, B, O and AB, respectively. 603(74.6%) participants passed the blood biosafety test, and 205(25.4%) failed due to unqualified IgG antibody titer(less than 160)(n=159) and positive infectious markers(n=46). Out of 1 699 bags of CP prepared, 1 566 were virus inactivated(bags of 100 mL, 150 mL and 200 mL specification constituted 642, 4 and 920, respectively) and 133 were non-inactivated(bags of 100 mL and 200 mL specification constituted 132 and 1, respectively).Conclusion The safety and therapeutic efficacy of CP prepared from recovered COVID-19 donors can be warranted by understanding the CP characteristics specific to COVID-19, standardizing the preparation procedures and employing effective safeguards.

关 键 词：新冠康复者血浆 血浆病毒灭活 无菌接驳 血浆速冻 

分 类 号：R373.1[医药卫生—病原生物学] R457.1[医药卫生—基础医学]