基于“热证可灸”理论研究艾灸对胃热证大鼠肠道微生态的影响  

Study on the effects of moxibustion on intestinal microecology of rats with stomach heat syndrome based on the theory of"moxibustion can be used on heat syndrome"

作　　者：余云进 谢宇锋 杨锦兰 杨宗保[2] 冯军 沈佳成 YU Yun-jin;XIE Yu-feng;YANG Jin-lan;YANG Zong-bao;FENG Jun;SHEN Jia-cheng(Shenzhen Hospital of Guangzhou University of Chinese Medicine(Futian),Shenzhen 518000,China;Medical College of Xiamen University,Xiamen 361102,China)

机构地区：[1]广州中医药大学深圳医院(福田),广东深圳518000 [2]厦门大学医学院,福建厦门361102

出　　处：《中国中医基础医学杂志》2020年第10期1470-1474,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：广东省中医药管理局科研项目(20191294)-基于“热证可灸”研究艾灸胃经穴通过脑肠互动促进胃热证大鼠胃黏膜损伤修复的作用机制;广东省自然科学基金资助项目(2017A030313748)-基于“热证可灸”研究艾灸胃经穴促进胃热证胃溃疡大鼠胃黏膜损伤修复的代谢组学调控机制;深圳市卫生计生委科研项目(201607061)-基于超高效液相色谱质谱联用技术研究艾灸促进胃热证胃溃疡大鼠胃黏膜损伤修复的靶效应蛋白;深圳福田区卫生公益性科研项目(FTWS20160033)-基于超高效液相色谱质谱联用技术研究艾灸促进胃热证胃溃疡大鼠胃黏膜损伤修复的靶效应蛋白。

摘　　要：目的:采用16S rDNA测序分析艾灸胃经穴对胃热证大鼠肠道微生态的影响。方法:24只SD大鼠按随机数字表法分为空白组、模型组、胃经穴组和对照点组各6只。运用Illumina Hiseq高通量测序技术分析胃热证大鼠和艾灸胃经穴干预胃热证大鼠的肠道菌群结构,利用LEfSe分析比较组间微生物的物种差异。结果:与空白组比较,模型组中红蝽菌科(Coriobacteriaceae)、柯林斯氏菌属(Collinsella)及梭菌属(Clostridium)等致病菌丰度显著上调。与模型组大鼠比较,胃经穴组中放线菌门(Actinobacteria)、双歧杆菌科(Bifidobacteriaceae)、双歧杆菌属(Bifidobacterium)和瘤胃球菌属(Ruminococcus)等益生菌丰度显著上调。结论:艾灸胃经穴“梁门”“足三里”能显著上调胃热证大鼠益生菌丰度水平,对大鼠的肠道微生物组成及物种影响具有调节作用,可能是其治疗胃热证的机制之一。Objective:To analyze the effect of moxibustion intervention for rats with stomach heat syndrome on gut microbiome by 16S rDNA sequencing.Methods:A total of twenty-four SD rats were randomly divided into four groups with six rats in each group,the normal group,the model group,the acupoint of stomach meridian group and the control point group.Illumina HiSeq sequencing was applied to analyze the microbial communities of rats.Differences in the gut microbiome of all groups were analyzed by LEfSe analysis.Results:Compared with the normal group,the abundance of pathogenic bacteria was significantly increased in the model group,such as Coriobacteriaceae,Collinsella and Clostridium.Compared with other groups,the abundance of probiotics bacteria was significantly increased in the acupoint of stomach meridian group,such as Actinobacteria,Bifidobacteriaceae,Bifidobacterium and Ruminococcus.Conclusion:Moxibustion at"Liangmen"and"Zusanli"of stomach meridian points can significantly increase the abundance level of probiotic bacterial in rats with stomach heat syndrome,and regulate the intestinal microbial composition and species of rats,which may be one of the mechanisms of its positive regulatory effect on the treatment of stmoach heat syndrome.

关 键 词：艾灸 胃热证 热证可灸 肠道微生态 足三里 梁门 神经降压素 

分 类 号：R245.81[医药卫生—针灸推拿学]