贵州小麦品种(系)粒重相关基因TaCwi-A1、TaSus2-2B和TaGW2-6A等位变异类型鉴定  

Identification and analysis of allele variation types of kernel weight related genes TaCwi-A1,TaSus2-2B and TaGW2-6A of Guizhou wheat varieties(lines)

作　　者：杨雪敏 李鲁华 任明见[1] 徐如宏[1] YANG Xue-min;LI Lu-hua;REN Ming-jian;XU Ru-hong(College of Agriculture,Guizhou University/Guizhou Branch of National Wheat Improvement Center,Guiyang 550025,China)

机构地区：[1]贵州大学农学院/国家小麦改良中心贵州分中心,贵阳550025

出　　处：《南方农业学报》2020年第9期2071-2081,共11页Journal of Southern Agriculture

基　　金：国家重点研发计划“七大农作物育种”重点专项(2017YFD0100900);贵州大学引进人才科研项目(贵大人基合字〔2017〕49)。

摘　　要：【目的】鉴定贵州小麦品种(系)中粒重相关基因TaCwi-A1、TaSus2-2B和TaGW2-6A的等位变异类型,筛选含高粒重基因型的小麦品种(系),为贵州小麦粒重的遗传改良和高粒重品种选育提供参考。【方法】以252份小麦品种(系)为材料,分别利用TaCwi-A1、TaSus2-2B和TaGW2-6A基因的分子标记(CWI22/CWI21、TaSus2-1/TaSus2-2和Hap-6A-P1/Hap-6A-P2)引物进行PCR扩增,利用毛细管电泳检测扩增产物,鉴定分析这3个基因的等位变异类型及分布频率,并结合籽粒性状测定结果,筛选含高粒重基因变异类型的小麦种质。【结果】252份小麦种质材料的粒重平均值为39.87 g,其中,有56份材料属于大粒种质(>45.00 g),仅有13份检测到等位变异;173份材料属于中粒种质(30.00~45.00 g),有24份检测到等位变异;23份材料属于小粒种质(<30.00 g),均未检测到等位变异。252份小麦材料中,含有TaCwi-A1、Ta Sus2-2B和TaGW2-6A基因等位变异的材料37份,占供试材料总数的14.7%,其中TaCwi-A1基因等位变异类型材料12份(包括TaCwi-A1a变异类型8份,Ta Cwi-A1b变异类型4份),占供试材料总数的4.8%;TaSus2-2B基因等位变异类型材料16份(包括TaSus2-2BH变异类型材料2份,Ta Sus2-2BL变异类型材料14份),占供试材料总数的6.4%;TaGW2-6A基因等位变异类型材料10份(包括Hap-6A-A变异类型4份,Hap-6A-G变异类型6份),占供试材料总数的4.0%;等位变异组合类型仅有1份材料(惠光2-2-2),为TaCwi-A1b/HAP-6A-A,占供试材料总数的0.4%。平均粒重最高的变异类型为TaCwi-A1b,其次是HAP-6A-G变异类型。对于平均粒重,TaCwi-A1b变异类型显著高于TaCwi-A1a变异类型(P<0.05,下同),Hap-6A-G变异类型显著高于Hap-6A-A变异类型,TaSus2-2BH变异类型也高于TaSus2-2BL变异类型,但差异不显著(P>0.05)。【结论】从贵州小麦品种(系)检测到的粒重基因等位变异整体较少,表明贵州小麦种质遗传多样性较低,高粒重品种的选育工作开展不够,今后应�【Objective】The allele variation types of kernel weight related genes TaCwi-A1,TaSus2-2 B and TaGW2-6 A in Guizhou wheat varieties(lines)were identified,and wheat varieties(lines)containing high kernel weight genotypes were screened to provide reference for genetic improvement of Guizhou wheat kernel weight and breeding of high kernel weight varieties.【Method】With 252 wheat varieties(or lines)as the materials,using molecular marker of genes TaCwiA1,TaSus2-2 B and TaGW2-6 A(CWI22/CWI21,TaSus2-1/TaSus2-2 and Hap-6 A-P1/Hap-6 A-P2)primers for PCR amplification,amplification products were detected using capillary electrophoresis,allele variation types and distribution of the three genes were identified,combined with the measuring results of grain traits,wheat germplasm with high kernel weight mutation types were screened.【Result】The average grain weight of 252 wheat germplasm materials was 39.87 g.Among them,56 materials belonged to large kernel germplasms(>45.00 g),and only 13 of them detected allele variation.Among the 173 medium kernel materials(30.00-45.00 g),allele variation was detected in 24.The rest 23 materials belonged to small kernel germplasm(<30.00 g),and no allele variation was detected.Among the 252 wheat materials,37 contained allelic variation of Ta Cwi-A1,TaSus2-2 B and TaGW2-6 Agenes,accounting for 14.7%of the total tested materials.Among them,12 contained allele variation of TacWI-A1 gene(including 8 of TaCwi-A1 a and 4 of TaCwi-A1 b),accounting for 4.8%of the total tested materials.There were 16 allele materials of TaSus2-2 B gene(including 2 allele materials of TaSus2-2 BH and 14 allele materials of Ta Sus2-2 BL),accounting for 6.4%of the total number of tested materials.There were 10 allele variation types of TAGW2-6 A(including 4 HAP-6 A-A variation types and 6 HAP-6 A-G variation types),accounting for 4.0%of the total number of materials tested.There was only one allele variation combination type of material(Huigang 2-2-2),which was Tacwi-A1 b/HAP-6 A-A,accounting for 0.4%of the to

关 键 词：小麦 粒重 基因 等位变异 分子标记 毛细管电泳 鉴定 

分 类 号：S512.103.53[农业科学—作物学]