参芪注射液对慢性阻塞性肺病大鼠氧化应激及免疫炎症反应的影响  

Effects of Shenqi injection on oxidative stress and immune inflammatory response in rats with COPD

作　　者：倪英 赵建 汪强涛 NI Ying;ZHAO Jian;WANG Qiang-tao(Department of Internal Medicine,Jingzhou Chest Hospital,Jingzhou Hubei 434000,China)

机构地区：[1]荆州市胸科医院内科,湖北荆州434000

出　　处：《临床和实验医学杂志》2020年第21期2252-2257,共6页Journal of Clinical and Experimental Medicine

基　　金：湖北省卫生厅科研基金资助一般项目(编号:QJX2012-46)。

摘　　要：目的分析参芪注射液对慢性阻塞性肺病(COPD)大鼠氧化应激及免疫炎症反应的影响,以期为COPD的临床干预提供实验依据及参考。方法50只无特定病原体(SPF)级雄性SD大鼠被随机分为模型组、高剂量组、中剂量组、低剂量组、正常对照组,每组各10只。采用被动吸烟法+气管内注射内毒素法制作COPD大鼠模型。制模成功后高、中、低剂量组分别按3.0、2.5、2.0 m L/(100 g·d)剂量给予参芪注射液,正常对照组给予等量0.9%氯化钠溶液。干预5周后采用AniRes2005动物肺功能分析系统比较5组大鼠肺功能[第零点3秒用力呼气容量(FEV0.3)/用力肺活量(FVC)、呼气峰流速(PEF)]、苏木精-伊红(HE)染色观察肺病理表现。比较5组大鼠氧化应激反应[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)]、免疫炎症[白细胞介素6(IL-6)、可溶性白细胞介素2受体(s IL-2R)、肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)]指标,蛋白免疫印迹法检测肺组织Toll样受体4(TLR-4)、核转录因子-κB(NF-κB)p65蛋白表达。结果①模型组肺组织HE染色可见不同程度肺病理改变,参芪注射液干预组大鼠肺组织病变明显改善,且高剂量组大鼠较模型组改善更为显著;与正常对照组比较,模型组FEV0.3/FVC、PEF均显著下降(P<0.05);与模型组比较,参芪注射液干预组FEV0.3/FVC、PEF均显著上升,且高剂量组显著高于中剂量组及低剂量组,差异有统计学意义(P<0.05);②与正常对照组比较,模型组MDA、IL-6、SIL-2R、TNF-α、IL-1β显著上升,GSH-Px、SOD显著下降(P<0.05);与模型组比较,参芪注射液干预组MDA、IL-6、SIL-2R、TNF-α、IL-1β水平显著下降,GSH-Px、SOD水平显著上升,且高剂量组MDA、IL-6、SIL-2R、TNF-α、IL-1β水平显著低于中剂量及低剂量组,GSH-Px、SOD水平显著高于中剂量及高剂量组,差异有统计学意义(P<0.05);③与正常对照组比较,模型组TLR-4、NF-κB p65蛋�Objective To analyze the effects of Shenqi injection on oxidative stress and immune inflammatory response in rats with chronic obstructive pulmonary disease(COPD),thus providing experimental basis and reference for clinical intervention of COPD.Methods Fifty SPF male Sprague-Dawley rats were randomly divided into the model group,the high-dose group,the middle-dose group,the low-dose group and the normal control group,with 10 rats in each group.Rat models of COPD were made by passive smoking and intratracheal injection of endotoxin.After successful modeling,the high-dose group,middle-dose group and low-dose group were treated with 3.0,2.5 and 2.0 m L/(100 g·d)of Shenqi injection,respectively.The model group and the normal control group were treated with the same volume of normal saline.After 5 weeks of intervention,the lung function[forced expiratory volume in 0.3 seconds(FEV0.3),forcedvital capacity(FVC),peak expiratory flow rate(PEF)]was compared among groups with AniRes2005 animal lung function analysis system,and pulmonary pathological manifestations were observed through hematoxylin-eosin(HE)staining.The oxidative stress[superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA]and immune inflammatory[interleukin 6(IL-6),soluble interleukin 2 receptor(s IL-2 R),tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β)]indexes were compared among groups.The protein expression of Toll-like receptor-4(TLR-4)and nuclear factorκB(NF-κB)p65 in lung tissues was detected by Western blotting.Results①HE staining of lung tissues of the model group showed different degrees of pulmonary pathological changes.Lung lesions of rats treated with Shenqi injection were significantly improved,and the improvement was more significant in the high-dose group than the model group.Compared with the normal control group,the FEV0.3/FVC and PEF of the model group were significantly decreased(P<0.05);compared with the model group,the FEV0.3/FVC and PEF of the Shenqi injection intervention group were significantly inc

关 键 词：大鼠 慢性阻塞性肺病 参芪注射液 氧化应激 免疫炎症反应 

分 类 号：R285.5[医药卫生—中药学]