过敏性哮喘患者高迁移率族蛋白1、Th1/Th2细胞亚群及相关细胞因子表达分析  

Analysis of high mobility group protein-1,Th1/Th2 cell subsets and the related cytokine expressions in patients with allergic asthma

作　　者：郝艳[1] 郑洪亮[1] HAO Yan;ZHENG Hong-liang(Department of Respiratory Medicine,Seventh People’s Hospital of Shenyang,Shenyang 110003,Liaoning,China)

机构地区：[1]沈阳市第七人民医院呼吸内科,沈阳110003

出　　处：《中国临床医学》2020年第5期837-840,共4页Chinese Journal of Clinical Medicine

摘　　要：目的:分析高迁移率族蛋白-1(high mobility group protein-1,HMGB-1)、辅助T淋巴细胞(Th)亚群以及相关细胞因子在不同严重程度的过敏性支气管哮喘患者中的表达意义。方法:选择98例轻中重度过敏性哮喘患者和30例同期体检的健康者(对照组),采用流式细胞术分析患者以及健康者外周血CD4^+干扰素-γ^+(IFN-γ^+)T细胞、CD4^+白细胞介素-4^+(IL-4^+)T细胞百分比。通过酶联免疫吸附测定法(ELISA)检测血清中HMGB-1、Th1细胞因子(IFN-γ)和Th2细胞因子(IL-4、IL-5)的表达水平。采用Spearman分析HMGB-1、Th1/Th2细胞亚群及其相关细胞因子与肺功能的相关性。结果:所有哮喘患者HMGB-1、CD4^+IL-4^+T细胞百分比、IL-4、IL-5均高于对照组;重度组患者HMGB-1、CD4^+IL-4^+T细胞百分比、IL-4、IL-5高于轻、中度患者。所有患者CD4^+IFN-γ^+T细胞百分比、IFN-γ表达均低于对照组;重度患者CD4^+IFN-γ^+T细胞百分比和IFN-γ表达低于轻、中度患者。HMGB-1、IL-4水平与肺功能负相关,IFN-γ水平与肺功能正相关。结论:HMGB-1和IFN-γ、IL-4可作为评价支气管哮喘严重程度的有效指标。Objective:To analyze the expression significance of high mobility group protein-1(HMGB-1),helper T lymphocyte(Th)subsets and related cytokines in patients with allergic asthma of different severity.Methods:A total of 98 patients with mild to moderate,severe allergic asthma and 30 healthy controls(control group)who underwent physical examination at the same time were selected.The percentages of CD4+interferon-γ+(IFN-γ+)T cells and CD4+interleukin-4+(IL-4+)T cells in peripheral blood of the patients and healthy controls were analyzed by flow cytometry.HMGB-1,Th1 cytokines IFN-γand Th2 cytokines IL-4,IL-5 levels in serum were detected by ELISA.The correlation between HMGB-1,Th1/Th2 cell subsets and their cytokines and severity of allergic asthma were analyzed by Spearman correlation analysis.Results:HMGB-1 expression,percentage of CD4+IFN-γ+T cells IL-4 and IL-5 expressions in all asthma patients were higher than those in the control group.The level of HMGB-1 protein,percentage of CD4+IL-4+T cells,IL-4 and IL-5 expressions in the severe group were higher than those in the mild and moderate group.The percentages of CD4+IFN-γ+T cells and IFN-γexpression in all patients were lower than those in the control group.The percentage of CD4+IFN-γ+T cells and IFN-γexpression in severe patients were lower than those in mild and moderate patients.Levels of HMGB-1 and IL-4 were negatively associated with lung function,while level of IFN-γwere positively associated with lung function.Conclusions:HMGB-1 and Th1/Th2 cytokines IFN-γand IL-4 could be used as effective indicators for evaluation of the severity of bronchial asthma.

关 键 词：哮喘 辅助T淋巴细胞1 辅助T淋巴细胞2 高迁移率族蛋白-1 

分 类 号：R562.25[医药卫生—呼吸系统]