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作 者:张淑颖 曲田丽[2] 李保华 ZHANG Shuying;QU Tianli;LI Baohua(College of Plant Health and Medicine;College of Chemistry and Pharmaceutical Sciences,Qingdao Agriculture University,Qingdao,Shandong 266109,China)
机构地区:[1]青岛农业大学植物医学学院 [2]青岛农业大学化学与药学院,山东青岛266109
出 处:《福建农林大学学报(自然科学版)》2020年第6期741-745,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基 金:青岛农业大学高层次人才科研基金(1116028);国家重点研发计划(2016YFD0201122)。
摘 要:采用SDS-PAGE法从剑麻内生枯草芽孢杆菌菌株JM-3无机发酵滤液中分离胞外蛋白,并测定了该蛋白对苹果树腐烂病菌的抑制作用.结果表明:枯草芽孢杆菌菌株JM-3以(NH_4)_2HPO_4为氮源时的抑菌活性最强;40%和50%饱和(NH_4)_2SO_4的盐析蛋白能显著抑制苹果树腐烂病菌菌丝生长,其中,分子质量约为4.5 ku的盐析蛋白能够抑制菌丝生长及分生孢子萌发,也可抑制离体苹果树枝条腐烂病斑扩展.The extracellular protein was isolated from cell-free fermentation liquid of Bacillus subtilis JM-3 produced by Agave sisalana using SDS-PAGE and its inhibition effects on Valsa ceratosperma were studied.The results showed that endophytic bacterium JM-3 could produce more antagonistic substance in inorganic medium with(NH4)2HPO4 as the nitrogen source.The proteins precipitated at 40%and 50%(NH4)2SO4 saturation could significantly inhibit the mycelial growth of V.ceratosperma.Among them,a 4.5 ku protein could inhibit the mycelial growth and conidial germination of V.ceratosperma significantly,and could inhibit canker lesion expansion on excised apple twigs.
分 类 号:S432.1[农业科学—植物病理学]
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