硒化镉量子点偶联羟基磷灰石荧光谱的温度特性  

Temperature Characteristics of Fluorescence Spectra of Cadmium Selenide Quantum Dots Coupled with Hydroxyapatite

作　　者：陈振宜 王锦添[1,3] 陈娜 刘书朋 王廷云[1,2,3] Chen Zhenyi;Wang Jintian;Chen Na;Liu Shupeng;Wang Tingyun(Key Laboratory of Specialty Fiber Optics and Optical Access Networks,Shanghai 200444,China;Shanghai Institute for Advanced Communication and Data Science,Shanghai 200444,China;Shanghai University,Shanghai 200444,China)

机构地区：[1]特种光纤与光接入网重点实验室,上海200444 [2]上海先进通信与数据科学研究院,上海200444 [3]上海大学,上海200444

出　　处：《中国激光》2020年第10期179-186,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(61575120,61475095);上海大学特种光纤与光接入网重点实验室开放项目(SKLSFO2018-05);高等学校学科创新引智计划(D20031)。

摘　　要：为了探究羟基磷灰石(HAP)纳米颗粒与硒化镉量子点(CdSe QD)的共轭物HAP-QD作为生物纳米温度探针的可能性,将CdSe QD与HAP纳米颗粒进行化学偶联,得到HAP-QD共轭物,并研究了HAP-QD荧光谱的温度特性。首先用硅烷偶联剂KH550对HAP表面进行氨基修饰;然后在偶联活化剂的作用下将表面修饰有羧基的CdSe QD与表面修饰有氨基的HAP进行共价偶联,得到HAP-QD共轭物;最后测量了298~318 K温度范围内CdSe QD和HAP-QD的荧光谱。实验结果表明,HAP-QD的荧光谱峰位随温度的升高会出现红移,且具有良好的线性关系。In order to study the possibility of the HAP-QD conjugate of hydroxyapatite (HAP) nanoparticles and cadmium selenide quantum dots (CdSe QD) as a biological nano-temperature probe, HAP nanoparticles are chemically coupled with CdSe QD to obtain HAP-QD conjugate, and further study the temperature characteristics of HAP-QD fluorescence spectrum. First, the surface of HAP is modified by silane coupling agent KH550. Then, under the action of coupling activator, the CdSe QD with a carboxyl group modified on the surface and HAP with an amino group modified on the surface are covalently coupled to obtain a HAP-QD conjugate. Finally, the fluorescence spectra of CdSe QD and HAP-QD are measured at the temperature range of 298--318 K. The experimental results show that the fluorescence peak position of HAP-QD demonstrates a redshift and has a good linear relationship with the increase of temperature.

关 键 词：光学传感 生物纳米温度探针 共价偶联 硒化镉量子点 羟基磷灰石 荧光温度测量 

分 类 号：O433.1[机械工程—光学工程]