铁皮石斛组培苗和栽培苗的circRNA检测及分析  

Detection and analysis of circRNA in tissue cultured seedlinog and cultivated seedling of Dendrobium officinale

作　　者：黄路瑶 郁吉锋 钱央央 徐涛[1] HUANG Luyao;YU Jifeng;QIAN Yangyang;XU Tan(College of Life Sciences and Medicine,Zhejiang Sci-tech University,HangZhou 310018,China)

机构地区：[1]浙江理工大学生命科学与医药学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2020年第6期840-845,共6页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省重点研发计划项目(2015C02030)。

摘　　要：为了分析铁皮石斛组培苗与有菌根的栽培苗的circular RNA (circRNA)变化,采用高通量测序方法对铁皮石斛组培苗和栽培苗进行circRNA检测,并分析它们之间的差异。结果显示:两组4个样品共测出4115组不同的circRNA,其中有显著性差异的有27种,在实验组中17种为显著上调,10种为显著下调;GO富集分析一共检测出99组数据,其中显著性基因最多是核基因;KEGG富集分析表明,circRNA共有富集在7条KEGG通路上,其中丁酸甲酯代谢途径(Butanoate metabolism)和错配修复途径(Mismatch repair)具有显著性差异。以上结果可为进一步研究铁皮石斛形成菌根前后circRNA的变化提供参考。In order to analyze the changes of circular RNA(circRNA) of Dendrobium officinale between the tissue cultured seedlings and the cultivated seedlings with mycorrhiza, the experiment was conducted to detect the circRNA of the tissue cultured seedlings and cultivated seedlings of D.officinale by high-throughput sequencing method, and the differences between them were analyzed. The results showed that 4115 groups of different circRNAs were detected in the four samples, of which 27 were significantly different. In the experimental group, 17 were significantly up-regulated and 10 were significantly down-regulated. A total of 99 sets of data were detected by GO enrichment analysis, of which the most significant genes were nucleus. KEGG enrichment analysis found that differential circRNAs were commonly enriched in 7 KEGG pathways and Butanoate metabolism and Mismatch repair had significant differences. The above results can provide a reference for further research on the changes of circRNA before and after the formation of mycorrhiza in D.officinale.

关 键 词：铁皮石斛 组培苗 栽培苗 circRNA 差异性表达 

分 类 号：Q94-3[生物学—植物学]