阿克苏某香猪场隐孢子虫和十二指肠贾第虫PCR检测  

PCR Detection of Cryptosporidium spp.and Giardia duodenalis in A Xiang Pig Farm of Aksu

作　　者：张盈 徐春艳 邢金明 敖维平 齐萌 井波[1] ZHANG Ying;XU Chun-yan;XING Jin-ming;AO Wei-ping;QI Meng;JING Bo(College of Animal Sciences,Tarim University/Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production and Construction Corps,Alar,Xinjiang,843300,China)

机构地区：[1]塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《动物医学进展》2020年第11期68-71,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31960704);新疆生产建设兵团中青年科技创新领军人才计划项目(2018CB034);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201806)。

摘　　要：为调查阿克苏某香猪场隐孢子虫和十二指肠贾第虫的感染情况,采集该场44份香猪新鲜粪便,提取基因组DNA,采用PCR进行检测和种类鉴定。结果发现,香猪隐孢子虫感染率为20.5%(9/44),断奶前和断奶后仔猪感染率分别为22.2%(2/9)和25.0%(7/28),成年母猪未发现感染,鉴定出猪隐孢子虫(n=2)与种母猪隐孢子虫(n=7);未发现贾第虫感染。检测结果为新疆地区香猪养殖业的健康发展提供了基础资料。To understand the infection rate of Cryptosporidium and Giardia duodenalis in Xiang pig,a total of 44 fresh fecal samples were collected,DNA extracted,and screened for the presence of these parasites by polymerase chain reaction(PCR)in a Xiang pig farm of Aksu.The infection rate of Cryptosporidium was 20.5%(9/44),with 22.2%(2/9)and 25.0%(7/28)in pre-weaned and post-weaned pigs,respectively.No positive sample was found in sows.Two Cryptosporidium species were identified,including C.suis(n=2)and C.scrofarum(n=7).Meanwhile,no positive sample of G.duodenalis was found in all samples.These results of this study provided a basic data for the health development of Xiang pig industry in Xinjiang.

关 键 词：隐孢子虫 十二指肠贾第虫 聚合酶链反应 香猪 

分 类 号：S852.72[农业科学—基础兽医学]