The general odorant receptor GmolOR9 from Grapholita molesta (Lepidoptera: Tortricidae) is mainly tuned to eight host-plant volatiles  

作　　者：Li-Hui Chen Ke Tian Gui-Rong Wang Xiang-Li Xu Ke-Hang He Wei Liu Jun-Xiang Wu 

机构地区：[1]State Key Laboratory of Crop Stress Biology in Arid areas,College of Plant Protection,Northwest A&F University,Yangling,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China

出　　处：《Insect Science》2020年第6期1233-1243,共11页昆虫科学（英文版）

基　　金：the National Key Research and Development Program of China(2018YFD0201400).

摘　　要：Grapholita molesta is one of the most destructive fruit pests distributed worldwide.Odorant receptors(ORs)located on the dendritic membrane of chemosensory neurons are deemed to be key molecules for sensing exogenous chemical signals.In this study,GmolOR9,a general OR from G.molesta,was functionally characterized.Quantitative real-time polymerase chain reaction revealed that GniolOR9 was more highly expressed in adults than in other stages,including eggs,larvae,and pupae.GmolOR9 expression was highly significantly more in the antennae of females than in those of males,and the highest level occurred in the antennae of 3-day-old female adults.GmolOR9 was broadly tuned to eight of 47 odorant components tested including(Z)-3-hexenyl acetate,butyl propionate,ethyl hexanoate,ethyl heptanoate,1-hexanol,(Z)-3-hexenol,2-ethyl-1-hexanol,and linalool,by in vitro heterologous expression.Furthermore,electroantennogram responses indicated that the efTects of dsOR9-injected females to(Z)-3-hexenyl acetate dramatically decreased.These results suggested that GmolOR9 might be involved in detecting hostplant volatiles.Moreover,(Z)-3-hexenyl acetate might serve as a potential attractant for the biological control of G.molesta.

关 键 词：Grapholita molesta oriental fruit moth odorant receptor VOLATILES 

分 类 号：Q96[生物学—昆虫学]