大豆抗胞囊线虫病潜在基因Meta-QTL和Overview分析  

Meta-QTL and Overview Analysis of Potential Genes Resistant to Cyst Nematode in Soybean

作　　者：王俊 姜海鹏 赵雪[1] 韩英鹏[1] WANG Jun;JIANG Hai-peng;ZHAO Xue;HAN Ying-peng(Soybean Research Insititute,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学大豆研究所,黑龙江哈尔滨150030

出　　处：《大豆科学》2020年第5期720-726,共7页Soybean Science

基　　金：国家自然基金面上项目(31671717,31971967);黑龙江省杰出青年基金(JC2018007);国家科技重大专项和重点研发项目(课题)省级资助项目(GX17B002);黑龙江省博士后项目(LBH-Q17015);国家重点研发计划(2016YFD0100304)。

摘　　要：为整合分析在不同遗传遗传群体中研究发现的数百个大豆胞囊线虫病抗性QTL,进一步挖掘大豆抗胞囊线虫病潜在基因,采用BioMercator 4.2软件将遗传背景和来源不同的QTL整合到大豆公共遗传图谱上,通过元分析法获取一致性QTL,并进行Overview分析。通过QTL元分析得到12个抗性候选QTL区段,主要分布在8、15、16、18和20号染色体上;通过Overview分析方法获得19个QTL,分别位于3、6、8、11、12、15、16、18和20号染色体上。2种方法挖掘到5个重叠QTL,共包含13个SCN抗性候选基因,6个LRR类型、2个LRR-TM类型和5个TIR-NBS-LRR类型抗性基因。QTL元分析和Overview分析方法可为抗大豆胞囊线虫病基因精细定位提供更确切的位点信息,进而为抗胞囊线虫病大豆分子辅助育种提供可靠的基因资源。In order to integrate and analyze hundreds of QTLs resistant to soybean cyst nematode(SCN) diseases found in different soybean genetic populations, to further explore the potential genes of soybean cyst nematode, we used BioMercator 4.2 software to integrate QTLs with different genetic backgrounds and project these QTL into soybean public genetic maps, and then, obtained consistent resistance QTLs through meta-analysis. In the same time we carried on Overview analysis. Finally, 12 resistance candidate QTLs were distributed on Chr 8, 15, 16, 18 and 20 through meta-analysis, 19 QTLs were located on Chr 3, 6, 8, 11, 12, 15, 16, 18 and 20 through Overview analysis. A total of 13 resistance genes were found in 5 overlapping QTLs through overlapping interval of the two methods, which covered 6 LRR type, 2 LRR-TM type, and 5 TIR-NBS-LRR type resistances disease genes. The QTL meta-analysis and Overview analysis method could provide more accurate genomic information for fine mapping, providing reliable gene resources for molecular assisted breeding of soybean resistant to cyst nematode.

关 键 词：大豆胞囊线虫 元分析 OVERVIEW 抗性位点 候选基因 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]