核桃壳提取液用于单针藻Monoraphidium sp.QLZ-3培养产油脂  

Production of lipid from walnut shell extracts by Monoraphidium sp.QLZ-3

作　　者：崔佳丽 游金坤[2] 李洁实 乔腾生 董训赞 Jiali Cui;Jinkun You;Jieshi Li;Tengsheng Qiao;Xunzan Dong(Yunnan Pharmaceutical Research Institute,Kunming 650111,Yunnan Province,China;All-China Federation of Supply and Marketing Cooperatives Kunming Institute of Edible Fungi,Kunming 650223,Yunnan Province,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,Yunnan Province,China)

机构地区：[1]云南省药物研究所,云南昆明650111 [2]中华全国供销合作总社昆明食用菌研究所,云南昆明650223 [3]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《微生物学报》2020年第11期2487-2497,共11页Acta Microbiologica Sinica

基　　金：云南省基础研究计划(2017FD038)。

摘　　要：【目的】研究核桃壳提取液(walnut shell extracts,WSE)对单针藻Monoraphidium sp.QLZ-3生长和油脂积累的影响。【方法】向BG-11培养基中添加不同量的WSE(培养基中保留有BG-11中全部营养成分)。【结果】结果显示,当BG-11培养基中的WSE含量为40%时,单针藻的生物量产率及油脂产率达到(534.70±4.07)mg/(L·d)和(296.35±15.36)mg/(L·d),相比对照组分别提高了的14.82%和33.50%,蛋白质和碳水化合物含量分别有不同程度的上调和下调。与对照组相比,微藻中谷胱甘肽(glutathione,GSH)和超氧化物歧化酶(superoxide dismutase,SOD)含量与活性均上调。此外,WSE作用下,微藻对多酚的移除达到84.37%,同时上调了核酮糖1,5-二磷酸羧化酶基因(ribulose 1,5-bisphosphate carboxylase/oxygenase,rbcL)和乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,accD)基因的表达量。【结论】研究表明,WSE联合BG-11可以提高微藻的生物量产率和油脂产率,降低微藻培养的原料成本,为核桃壳的资源化利用及微藻的工业化生产提供了一定的技术支撑。[Objective]The effects of walnut shell extracts(WSE)on the growth and lipid accumulation of Monoraphidium sp.QLZ-3 were explored.[Methods]Different volumes of WSE were added to BG-11 medium(all nutrients in BG-11 were retained in the medium).[Results]The biomass and lipid productivity reached(534.7±4.07)mg/(L·d)and(296.4±15.36)mg/(L·d)with addition of 40%WSE in the culture medium,increasing 14.82%and 33.50%,respectively,with upregulation of protein and downregulation of carbohydrate.[Conclusion]Exogenous WSE could enhance the biomass and lipid productivities in microalgae.

关 键 词：核桃壳提取液 Monoraphidium sp. QLZ-3 活性氧 生物量产率 油脂产率 

分 类 号：Q949.9[生物学—植物学]