Tomography-assisted Doppler photoacoustic microscopy:proof of concept  

作　　者：Xinkun Wang Kedi Xiong Xin Jin Sihua Yang 王新坤;熊科迪;金鑫;杨思华(MOE Key Laboratory of Laser Life Science&Inst itute of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou 510631,China;Guangdong Provincial Key Laboratory of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou 510631,China)

机构地区：[1]MOE Key Laboratory of Laser Life Science&Inst itute of Laser Life Science,College of Biophotenics,South China Normal University,Guangzhou 510631,China [2]Guangdong Provincial Key Laboratory of Laser Life Science,Collge of Biophotonics,South China Normal University,Guangzhou 510631,China

出　　处：《Chinese Optics Letters》2020年第10期53-57,共5页中国光学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 61822505,11774101,61627827,and 81630046);the Science and Technology Planning Project of Guangdong Province,China(No. 2015B020233016);the China Postdoctoral Science Foundation (No. 2019M652943);the Natural Science Foundation of Guangdong Province,China(No. 2019A1515011399);the Science and Technology Program of Guangzhou (No. 2019050001)

摘　　要：The previous methods to measure flow speed by photoacoustic microscopy solely focused on either the transverse or the axial flow component, which did not reflect absolute flow speed. Here, we present absolute flow speed maps by combining Doppler bandwidth broadening with volumetric photoacoustic microscopy. Photoacoustic Doppler bandwidth broadening and photoacoustic tomographic images were applied to measure the transverse flow component and the Doppler angle, respectively. Phantom experiments quantitatively demonstrated that ranges of 55° to 90° Doppler angle and 0.5 to 10 mm/s flow speed can be measured. This tomography-assisted method provides the foundation for further measurement in vivo.

关 键 词：medical optics and biotechnology photoacoustic imaging scanning microscopy flow speed 

分 类 号：TH742[机械工程—光学工程]