Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins  

作　　者：Hongwei Sun Shengyao Zhi Guifang Wu Guanglan Wu Tianqi Cao Hu Hao Zhou Songyang Puping Liang Junjiu Huang 

机构地区：[1]MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research,SYSU-BCM Joint Research Center,School of Life Sciences,Sun Yat-sen University,Guangzhou,510275,China [2]Department of Paediatrics,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou,510655,China [3]Key Laboratory of Reproductive Medicine of Guangdong Province,School of Life Sciences,and the First Affiliated Hospital,Sun Yat-sen University,Guangzhou,510275,China [4]State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou,510060,China [5]Verna and Marrs Mclean Department of Biochemistry and Molecular Biology,Baylor College of Medicine,One Baylor Plaza,Houston,TX,77030,USA

出　　处：《Journal of Genetics and Genomics》2020年第6期337-340,共4页遗传学报（英文版）

基　　金：supported by the National Key R&D Program of China(2017YFC1001901,2017YFA0102801 and 2017YFC1001603);the National Natural Science Foundation of China(91640119,31671540,81330055 and 31601196);the Guangdong Special Support Program(2019BT02Y276);the Natural Science Foundation of Guangdong Province(2016A030310206 and 2014A030312011);the Science and Technology Planning Project of Guangdong Province(2015B020228002);the Guangzhou Science and Technology Project(201707010085 and 201803010020)。

摘　　要：More than 32,000 pathogenic single nucleotide polymorphisms(SNPs)have been identified in the human genome(Gaudelli et al.,2017).Genetically modified mice with pathogenic SNPs are good models for studies of disease pathogenesis and the development of new therapeutics.Accordingly,an efficient,high-throughput method for the generation of mouse models with SNPs is needed.

关 键 词：al. EDITOR pathogenesis 

分 类 号：Q78[生物学—分子生物学]