骨髓间充质干细胞促进急性脊髓损伤大鼠脊髓组织神经元修复作用的研究  

Bone marrow mesenchymal stem cells promote nerve cell repair in spinal cord tissue of rats with acute spinal cord injury

作　　者：贾宇涛[1] 刘洋[1] 孙天威[1] 田融[1] Jia Yutao;Liu Yang;Sun Tianwei;Tian Rong(Department of Spine Surgery,Tianjin Union Medical Center,Tianjin 300121,China)

机构地区：[1]天津市人民医院脊柱外科,300121

出　　处：《国际生物医学工程杂志》2020年第4期269-274,303,共7页International Journal of Biomedical Engineering

基　　金：天津市自然科学基金(18JCYBJC28200);天津市人民医院科研基金(2019JZPY06)。

摘　　要：目的探讨尾静脉移植骨髓间充质干细胞(BMSCs)对急性脊髓损伤大鼠脊髓组织神经元的修复作用。方法将60只Sprague-Dawley雄性大鼠按随机数字表法分为假手术组、模型组和干细胞组,每组20只。模型组和干细胞组均采用改良的Allen′s打击法构建急性脊髓损伤大鼠模型;假手术组不进行脊髓打击损伤,只行手术暴露。模型建立24 h后,干细胞组大鼠尾静脉注射0.2 ml BMSCs单细胞悬液(2×106个细胞);假手术组及模型组大鼠尾静脉注射同体积的氯化钠注射液。采用Basso-Beattie-Bresnahan(BBB)评分法评价造模后第1、4、7、15、30天大鼠的后肢运动功能。造模后第30天,采用酶联免疫测定法检测大鼠脊髓组织中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和前列腺素E2(PGE2)的含量变化,苏木精-伊红染色观察大鼠脊髓组织的病理学变化,尼氏染色分析大鼠脊髓组织中尼氏小体与神经元的变化。结果相对于模型组[(1.82±0.84)、(3.38±0.88)、(5.83±1.36)分],干细胞组大鼠造模后第7、15、30天的BBB评分[(5.68±0.82)、(10.25±1.55)、(13.25±2.36)分]均明显升高,差异均具有统计学意义(均P<0.01)。造模后第30天,与模型组相比,干细胞组大鼠脊髓组织中的TNF-α、IL-1β和PGE2含量均明显降低(均P<0.01),脊髓组织损伤明显减轻,神经元及尼氏小体数量均明显升高(均P<0.01)。结论尾静脉移植BMSCs可显著改善大鼠急性脊髓损伤,其可能是通过调控炎症因子TNF-α、IL-1β和PGE2的表达加快脊髓组织神经元的修复,进而促进急性脊髓损伤大鼠运动功能的恢复。Objective To study the repair effects of bone marrow mesenchymal stem cells(BMSCs)injection via the caudal vein on the nerve cells in the spinal cord tissue of rats with acute spinal cord injury.Methods Sixty Sprague-Dawley male rats were divided into sham operation group,model group and BMSCs group using the random number table method,with 20 rats in each group.The Allen's method was used in the model group and BMSCs group to construct the rat models of a spinal cord injury model.Rats in the sham operation group did not undergo spinal cord injury and only received surgical exposure.24 hours after the establishment of the model,rats in the BMSCs group were received 0.2 ml BMSCs single cell suspension(2×106 cells)via tail vein injection.Rats in the sham operation group and model group were received the same volume of 0.2 ml Sodium chloride solution via tail vein injection.The motor function of the rats on the 1st,4th,7th,15th and 30th day after modeling was recorded by Basso-Beattie-Bresnahan(BBB)scoring method.The contents of inflammatory factors tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-β)and Prostaglandin E2(PGE2)in spinal cord tissue of rats were detected by enzyme-linked immunosorbent assay(ELASA)on the 30th day after modeling.Hematoxylin-eosin staining was used to observe the pathological changes of rat spinal cord tissue.Nissl staining was used to analyze the changes of Nissl bodies and neuron cells in rat spinal cord tissue.Result Compared with the model group,the BBB scores of the BMSC group were significantly increased on the 7(5.68±0.82 vs 1.82±0.84),15(10.25±1.55 vs 3.38±0.88)and 30(13.25±2.36 vs 5.83±1.36)days after modeling,and the differences were statistically significant(all P<0.01).Compared with the model group,the levels of TNF-α,IL-1βand PGE2 in the spinal cord tissue of the BMSCs group were significantly lower than those in the model group on the 30 days after modeling(all P<0.01).Besides,the spinal cord tissue injury was significantly reduced,and the number of neurons a

关 键 词：脊髓损伤 间质干细胞移植 神经元 炎症因子 运动功能 

分 类 号：R651.2[医药卫生—外科学]