氯化钠胁迫对甜菜幼苗伤害和抗氧化酶系统的影响  

Effects of sodium chloride stress on injury and antioxidant system of sugar beet seedlings

作　　者：刘钰 孙学伟[1] 於丽华 王堽 耿贵 LIU Yu;SUN Xuewei;YU Lihua;WANG Gang;GENG Gui(College of Life Science,Heilongjiang University,Harbin 150080,China;Crop Research Institute,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080 [2]黑龙江大学农作物研究院,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2020年第5期588-593,共6页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(31701487);国家糖料产业技术体系资助项目(CARS-170209)。

摘　　要：为了解NaCl胁迫对甜菜幼苗不同阶段的危害差异,选用不同品系甜菜幼苗在子叶期、第一对真叶期和第三对真叶期开始中度和重度NaCl胁迫,分析NaCl胁迫对叶片伤害和抗氧化酶系统的影响。采用室内营养液培养方法,分析NaC处理的不同时期耐盐品系T510和盐敏感品系S210甜菜幼苗叶片透性、丙二醛(MDA)含量及抗氧化酶活性的变化。结果表明:(1)NaCl处理提高了甜菜幼苗叶片胞膜透性和MDA含量,以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GPX)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)的活性,且上述指标随着NaCl胁迫浓度的提高呈现明显增加,其中APX酶活性提高幅度最大;(2)随着甜菜幼苗苗龄的增长,NaCl处理的甜菜幼苗叶片胞膜透性和MDA含量的提高幅度逐渐降低,SOD、GPX、CAT和APX活性增加幅度也逐步降低;(3)在同一苗龄,NaCl胁迫下盐敏感品系S210比耐盐品系T510积累更多MDA,胞膜透性增大,抗氧化酶防御酶活性降低;(4)子叶期的盐害指数明显高于一对真叶期和三对真叶期,盐敏感品系的盐害指数明显高于耐盐品系。因此,NaCl浓度的升高加重幼苗细胞膜伤害,不断提高抗氧化防御能力;随着甜菜幼苗苗龄的增长,幼苗细胞膜的盐害逐渐减轻,抗氧化防御能力逐渐降低;盐敏感品系S210在NaCl胁迫下抗氧化防御能力弱,受到盐胁迫伤害较重。In order to explore the effect of NaCl stress on sugar beet seedlings at different stages,different cultivars of sugar beet seedlings were selected to treat with moderate and severe NaCl stress at cotyledon stage,first true leaf stage and third true leaf stage.Moreover,the effects of NaCl stress on leaf injury and antioxidant enzyme system were analyzed.The changes of leaf permeability,malondialdehyde(MDA)content and antioxidant enzyme activity in the leaves of salt tolerant cultivar T510 and salt sensitive cultivar S210 were analyzed in this study.Firstly,the results showed that NaCl treatment increased the membrane permeability and MDA content,and the activities of superoxide dismutase(SOD),glutathione peroxidase(GPX),catalase(CAT)and ascorbic acid peroxidase(APX)in the leaves of sugar beet were increased.Furthermore,the above indexes increased significantly with the increase of NaCl stress concentration,and the activity of APX increased most.Secondly,with the increase of seedling age,the increase of membrane permeability and MDA content in leaves of sugar beet seedlings treated with NaCl decreased gradually,and the activities of SOD,GPX,CAT and APX also decreased gradually.Thirdly,under NaCl stress the salt sensitive cultivar S210 accumulated more MDA than the salt tolerant cultivar T510,and S210 increased the membrane permeability and decreased the activities of antioxidant enzymes and defense enzymes compared with T510.Lastly,the salt damage index of cotyledon stage was significantly higher than that of one pair of true leaf stage and three true leaf stage,and the salt injury index of salt sensitive strain was significantly higher than that of salt tolerant strain.Therefore,the increase of NaCl level aggravated the injury of cell membrane and enhanced the ability of antioxidant defense.With the growth of seedling age,the salt damage of seedling cell membrane was gradually reduced,and the antioxidant defense ability was gradually reduced.The salt sensitive strain S210 had weak antioxidant defense ability unde

关 键 词：甜菜 盐胁迫 抗氧化系统 膜系统 

分 类 号：S566.3[农业科学—作物学]