新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异  

Comparison of Ethanolic Extract of Wild Artemisia rupestris L.Removing Polysaccharides on DCs Maturation

作　　者：张爱莲[1] 杨雨[1] ZHANG Ailian;YANG Yu(School of Life Science and Technology,Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Xinjiang University,Urumqi Xinjiang 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源与基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《新疆大学学报（自然科学版）》2020年第4期508-514,共7页Journal of Xinjiang University(Natural Science Edition)

基　　金：国家自然科学基金(31360224,31660259).

摘　　要：比较新疆一枝蒿未除多糖醇提物(Ethanol extract of wild Artemisia rupestris L.,EEWAR)和除多糖醇提物(Ethanol extract of wild Artemisia rupestris L removing polysaccharides,DEEWAR)的免疫调节活性.利用体外树突状细胞(Dendritic cells,DCs)为平台,FACS(Fluorescence Activating Cell Sorter)技术检测未除多糖EEWAR和除多糖EEWAR对DCs成熟的影响.超声法提取EEWAR,检测其多糖和黄酮含量.C57BL/6小鼠骨髓细胞进行体外培养,第7 d后收获DCs,不同剂量未除多糖及除多糖的EEWAR分别刺激DCs细胞12 h后,LPS为阳性对照,检测DCs表面分子(CD11c,CD40,CD80,CD86和MHCII)表达情况.未除多糖和除多糖的EEWAR多糖含量分别为30.1%,5.3%,黄酮含量分别为5.6%,16.4%.FACS实验结果显示,未除多糖及除多糖的EEWAR均能够剂量依赖性促进CD80,CD86和MHCII分子的表达水平,且除多糖后促进DCs成熟的能力增强了约一倍;未除多糖及除多糖的EEWAR对CD40分子的促进作用不明显.结果表明,EEWAR除多糖后免疫调节活性明显增强.To compare the immunoregulatory activities of ethanol extract of wild Artemisia rupestris L.,(EEWAR)and ethanol extract of wild Artemisia rupestris L.(DEEWAR)removing polysaccharides.Using Dendritic cells(Dendritic cells,DCs)as a platform in vitro,The effects of EEWAR with polysaccharide or polysaccharide removal on DCs maturtion were detected by Fluorescence Activating Cell Sorter(FACS).EEWAR was extracted by ul-trasonic method and its content of sugar and flavonoid was detected.Marrow-derived cells from C57BL/6 mice were cultured in vitro,and DCs were harvested on day 7 after culture.DCs were stimulated for 12h by EEWAR with different doses,respectively.Expression of DCs surface molecules(CD11c,CD40,CD80,CD86 and MHCII)were detected.The content of polysaccharides and flavonoid of EEWAR was 30.1%,5.3%,5.6%and 16.4%,re-spectively.FACS results showed that EEWAR with polysaccharide or polysaccharide removal could increase the expression levels of CD80,CD86 and MHCII molecules in a dose-dependent manner,and the ability to promote DCs maturation after removing polysaccharides was doubled.The effect of EEWAR on CD40 was not obvious.The results showed that the immunomodulatory activity of EEWAR was significantly enhanced after the removal of polysaccharides.

关 键 词：一枝蒿 醇提物 DCS 免疫调节 

分 类 号：R392.11[医药卫生—免疫学]