克罗恩病患者血浆中鸟甘与尿鸟苷水平的临床研究  

Clinical study on plasma levels of guanylin and uroguanylin in patients with Crohn disease

作　　者：徐东燕 张晓雨[2] 赵海剑[2] 王健[2] 孙静[3] Xu Yandong;Zhang Xiaoyu;Zhao Haijian;Wang Jian;Sun Jing(Department of Gastroenterology,Second People′s Hospital of Huai′an,Jiangsu Province,Huai′an 223002,China;Department of Gastrointestinal Surgery,Second People′s Hospital of Huai′an,Jiangsu Province,Huai′an 223002,China;Department of Gastroenterology,Wuxi People′s Hospital Affiliated to Nanjing Medical University,Wuxi 214000,China)

机构地区：[1]淮安市第二人民医院消化内科,江苏省223002 [2]淮安市第二人民医院胃肠外科,江苏省223002 [3]南京医科大学附属无锡市人民医院消化内科,江苏省214000

出　　处：《中华消化病与影像杂志（电子版）》2020年第6期248-251,共4页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

基　　金：国家自然科学基金(81800490);江苏省“333”高层次人才项目(BRA2017247);江苏省六大人才高峰项目(WSW-220)。

摘　　要：目的评价克罗恩病(CD)患者血浆鸟苷前体激素(ProGN)和尿鸟苷前体激素(ProUGN)水平在临床中的价值。方法采用酶联免疫吸附试验(ELISA)对克罗恩病患者和健康对照者血浆ProGN和ProUGN水平进行检测,并分析其与临床疾病活动性及24 h大便次数的相关性。结果与健康对照者相比,CD患者血浆ProGN与ProUGN水平均明显降低(均P<0.05)。亚组分析显示,缓解期与活动期患者血浆ProGN水平均显著低于健康对照组(均P<0.001),活动期患者水平低于缓解期患者,但差异无统计学意义(P=0.121);缓解期患者血浆ProUGN水平显著低于健康对照组(P<0.001),活动期患者低于健康对照组,但差异无统计学意义(P=0.266);缓解期患者显著低于活动期患者,差异有统计学意义(P=0.011)。Pearson相关性分析显示,血浆ProGN及ProUGN水平与患者24 h大便次数均呈显著负相关(r=-0.804与r=-0.767,均P<0.001)。血浆ProGN水平与Best克罗恩病活动指数呈显著负相关(r=-0.561,P<0.001);而ProUGN水平与Best克罗恩病活动指数无线性相关(r=-0.155,P=0.314)。结论克罗恩病患者疾病临床活动性和腹泻可能是血浆ProGN水平的决定因素。Objective To evaluate the clinical value of ProGN and ProUGN in patients with Crohn disease(CD).Methods The plasma ProGN and ProUGN levels of Crohn disease patients and healthy controls were detected by enzyme-linked immunosorbent assay(ELISA),and the correlation between plasma levels and clinical disease activity and 24-hour stool frequency was analyzed.Results Compared with the healthy controls,the plasma ProGN and ProUGN levels of CD patients were significantly lower(P<0.05).Subgroup analysis showed that the plasma ProGN level of patients in remission period and active period was significantly lower than that of healthy control group(P<0.001),while the level of patients in active period was lower than that of patients in remission period,but there was no statistical significance(P=0.121);the plasma ProUGN level of patients in remission period was significantly lower than that in healthy control group(P<0.001),and plasma level of patients in active period was lower than that in healthy control group,but there was no statistical significance(P=0.266),and plasma level of patients in remission period was significantly lower than that in active period(P=0.011).Pearson correlation analysis showed that plasma ProGN and ProUGN levels were negatively correlated with 24-hour stool frequency(r=-0.804,P<0.001;r=-0.767,P<0.001).There was a significant negative correlation between the plasma ProGN level and the Best Crohn disease activity index(r=-0.561,P<0.001),but there was no linear correlation between the plasma ProGN level and the Best Crohn disease activity index(r=-0.155,P=0.314).Conclusion The clinical activity and diarrhea may be the determinants for level of proGN in plasma.

关 键 词：克罗恩病 鸟甘 尿鸟苷 

分 类 号：R47[医药卫生—护理学]