靶向抑制CDK6对人卵巢癌细胞凋亡的影响  

The Effect of Targeted CDK6 Inhibition on the Apoptosis of Human Ovarian Cancer Cell

作　　者：段丽[1] 王莉莉[1] 冯雪[2] 王冠[1] 邢焱玲 孙杰[1] 颜莹[3] DUAN Li;WANG Lili;FENG Xue;WANG Guan;XING Yanling;SUN Jie;YAN Ying(Department of Medical Education,Heilongjiang Provincial Hospital,Harbin Heilongjiang 150036,China;Department of Reproductive Medicine,The First Affiliated Hospital of Harbin Medical University,Harbin Heilongjiang 150001,China;Department of Oncology,Harbin First Hospital,Harbin Heilongjiang 150001,China)

机构地区：[1]黑龙江省医院医教科,黑龙江哈尔滨150036 [2]哈尔滨医科大学附属第一医院生殖医学科,黑龙江哈尔滨150001 [3]哈尔滨市第一医院肿瘤科,黑龙江哈尔滨150001

出　　处：《中国卫生标准管理》2020年第21期130-132,共3页China Health Standard Management

基　　金：黑龙江省卫生健康委科研课题(2018326)。

摘　　要：目的探讨抑制卵巢癌细胞周期蛋白依赖性激酶(cyclindependent kinases,CDK6)基因后人卵巢癌细胞凋亡变化。方法将特异性CDK6-shRNA-608重组质粒和无关系列载体质粒转染人HO-8910卵巢癌细胞,并设立空白对照组;采用实时定量反转录PCR(real-time RT-PCR)方法检测转染后48 h各组HO-8910细胞CDK6 mRNA表达情况;流式细胞束检测各组HO-8910细胞凋亡的变化。结果特异性shRNA-608重组质粒转染HO-8910细胞48 h时CDK6 mRNA表达受到明显抑制,shRNA-608组细胞的凋亡率显著增加,与无关序列转染组和空白对照组相比,差异有显著性统计学意义(P<0.01)。结论抑制CDK6基因可促进卵巢癌HO-8910细胞的凋亡。Objective To investigate the effect of CDK6 gene inhibition on the apoptosis of human ovarian cancer HO-8910 cells.Methods Specific CDK6-shRNA-608 recombinant plasmid and unrelated sequence shRNA were transfected in human HO-8910 cells,and the normal cells was set up as a blank control group.The expression of CDK6 mRNA in HO-8910 cells in different groups was detected by real-time RT-PCR at 48 hours post transfection.The apoptosis of HO-8910 cells in different groups were detected by FACS.Results Expression of CDK6 gene mRNA in HO-8910 cells was significantly inhibited by CDK6 shRNA-608 at 48 hours post transfection.The cell apoptosis increased significantly in shRNA-608 group,compared with the unrelated sequence group and the blank control group,the difference was significant(P<0.01).Conclusion CDK6 gene inhibition can promote apoptosis of HO-8910 cells in ovarian cancer.

关 键 词：卵巢癌细胞 CDK6 抑制 SHRNA HO-8910细胞 凋亡 

分 类 号：R3[医药卫生—基础医学]