抗肿瘤多肽的高效筛选方法及研究现状  

Advances in effectively screening anti-tumor peptides

作　　者：沈莉莉 王姣姣 陈吴越 周婧 马宏跃 许惠琴 程海波 陆茵 段金廒 SHEN Li-li;WANG Jiao-jiao;CHEN Wu-yue;ZHOU Jing;MA Hong-yue;XU Hui-qin;CHENG Hai-bo;LU Yin;DUAN Jin-ao(Jiangsu Provincial Key Laboratory of High-Tech Research for Formulations/Jiangsu Provincial Collaborative Innovation Center of Chinese Medicine Resources Industrialization Process,School of Pharmacy,Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China;Jiangsu Provincial Key Laboratory of Efficacy and Safety of Traditional Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center for Traditional Chinese Medicine Prevention and Treatment of Cancer,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心,江苏南京210023 [2]江苏省中药药效与安全性重点实验室,江苏南京210023 [3]江苏省中医药防治肿瘤协同创新中心,江苏南京210023

出　　处：《药学学报》2020年第10期2298-2305,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81673563,81274199);江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心资助项目(FJGJS-2015-15,ZDXM-14);江苏省青蓝工程学术带头人项目。

摘　　要：恶性肿瘤是严重危害人类健康的疾病,常规抗肿瘤的化疗药物存在易耐药和不良反应的问题。而天然毒液肽和人工设计的靶向多肽分子质量小,易到达或亲和于肿瘤组织,具有较好的抗癌活性和成药性,其发现、筛选和鉴定方法引起大家广泛关注。本文首先列举了近年来热点研发的抗肿瘤多肽类型,进而介绍了基于传统色谱分离、肽组学、噬菌体展示、生物表型以及人工智能技术的活性肽、靶向肽筛选原理、方法和实例应用,以期为未来新型抗肿瘤多肽的研发提供方法学借鉴和参考。Malignant tumor is a disease that severely threaten human health.Common chemotherapeutical drugs currently used in clinical practice have some problems in severe side effects and chemoresistance.In contrast,natural venom peptides and artificially designed targeting peptides have excellent biological activities and potential druggability due to their small molecular weights and high affinity to tumor tissues.Thus,the methods for the discovery of anti-tumor peptides have attracted much attention.In this paper,we summarized the types of anti-tumor peptides from recent literatures.Then,we systematically reviewed screening theories,methods and applications based on traditional chromatographic separation,peptidomics,phage display,phenotypic screening,and artificial intelligence.These strategies and technologies will provide a methodological reference for accelerating anti-tumor peptides research.

关 键 词：抗肿瘤多肽 色谱分离 肽组学 噬菌体展示技术 表型筛选 

分 类 号：R966[医药卫生—药理学]