款冬花总倍半萜对OVA致敏哮喘大鼠干预作用的代谢组学  

The effect of the sesquiterpenes from Flos Farfarae on an OVA-sensitized asthma model in rats

作　　者：段亚辉 秦雪梅[1] 李震宇[1] DUAN Ya-hui;QIN Xue-mei;LI Zhen-yu(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《药学学报》2020年第10期2414-2420,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(31270008,81973466);国家中药标准化项目(ZYBZH-Y-JIN-34);山西省科技创新重点团队(201605D131045-18);地产中药功效物质研究与利用山西省重点实验室(201605D111004)。

摘　　要：哮喘是由嗜酸性粒细胞、肥大细胞、淋巴细胞、中性粒细胞等多种炎症细胞和细胞组分参与的慢性炎症疾病。中医药在哮喘治疗方面具有独特的优势。本研究拟采用卵清蛋白(OVA)诱导大鼠哮喘模型分析款冬花总倍半萜对哮喘的干预作用。支气管肺泡灌洗液(BALF)细胞计数、肺组织病理切片、血清炎症因子等结果显示,款冬花总倍半萜具有明显的抗哮喘作用,代谢组学分析结果显示,哮喘动物模型中有25个代谢物发生改变,款冬花总倍半萜可以回调其中18个,其机制可能在于款冬花总倍半萜阻断p65 NF-κB过度激活,阻断促炎因子信号向胞内的传递,减少炎症细胞的过度激活,从而抑制炎症因子分泌,进而缓解哮喘炎症。本研究为进一步的抗哮喘中药新药研发奠定了基础,但款冬花总倍半萜对哮喘的作用机制还需要进一步的深入研究。Asthma is a chronic inflammatory disease involving eosinophils,mast cells,lymphocytes,neutrophils and other inflammatory cells and immune components.Chinese medicine has unique advantages in the treatment of asthma.In this study an ovalbumin(OVA)-induced asthma model in rats was used to verify the effect of the total sesquiterpenoids of Flos Farfarae(F.F.S)on asthma.The results show that the F.F.S has anti-asthmatic effects.Metabolomic analysis showed that 25 metabolites were changed in asthmatic rats,and 18 of them could be attributed to F.F.S.F.F.S.can block the over-activation of p65 NF-κB,preventing the transmission of pro-inflammatory factor signals to cells.F.F.S.thus could reduce the over-activation of inflammatory cells,inhibiting the secretion of inflammatory factors and further alleviating asthma inflammation.This study lays a foundation for further new drug research.The mechanism of F.F.S.on asthma needs further investigation.

关 键 词：哮喘 款冬花 倍半萜 作用机制 代谢组学 

分 类 号：R917[医药卫生—药物分析学]