福建省龙岩市333份分枝杆菌菌种鉴定结果回顾性分析  

Retrospective analysis of the identification results of 333 Mycobacterium species in Longyan City,Fujian Province

作　　者：黄文滨 张满娥[1] 卢志华 邓文华 张洪彬[1] HUANG Wenbin;ZHANG Man'e;LU Zhihua;DENG Wenhua;ZHANG Hongbin(Department of Molecular Biology Laboratory,the Second Hospital of Longyan,Longyan,Fujian 364000,China)

机构地区：[1]福建省龙岩市第二医院分子生物实验室,福建龙岩364000

出　　处：《检验医学与临床》2020年第22期3275-3278,共4页Laboratory Medicine and Clinic

摘　　要：目的了解龙岩市分枝杆菌感染的阳性率及分枝杆菌菌种分布情况,为龙岩市结核病及非结核分枝杆菌病的防治及流行病学研究提供科学依据。方法采用DNA微阵列芯片法检测来该院就诊的756例疑似结核病患者的标本,并进行临床上常见的17种分枝杆菌的检测,计算分枝杆菌感染的阳性率及分枝杆菌菌种的分布特点。结果756份标本中分枝杆菌阳性333份(44.05%),其中结核分枝杆菌复合群阳性290份(87.09%),非结核分枝杆菌阳性43份(12.91%),非结核分枝杆菌种群分布达10种,以胞内分枝杆菌(25.58%)、鸟分枝杆菌(20.93%)、龟或脓肿分枝杆菌(18.60%)菌种为主,有7种非结核分枝杆菌未被检出。结论龙岩市分枝杆菌菌种分布广泛,分枝杆菌感染以结核分枝杆菌为主,非结核分枝杆菌感染以胞内分枝杆菌、鸟分枝杆菌、龟或脓肿分枝杆菌为主。分枝杆菌菌种鉴定对结核病及非结核分枝杆菌病的诊断及治疗有较好的指导意义。Objective To understand the positive rate of Mycobacterium infection and the distribution of Mycobacterium species in Longyan City,and provide a scientific basis for prevention and treatment and epidemiological study of tuberculosis and nontuberculosis in Longyan City.Methods The DNA microarray chip was used to detect the 756 specimens who suspected tuberculosis in the hospital,to detect the 17 common kinds of Mycobacteria in clinical.Results Totally 333(44.05%)specimens out of 756 specimens were positive,among which 290(87.09%)specimens were detected as Mycobacterium tuberculosis complex group,while 43(12.91%)specimens were detected as nontuberculosis Mycobacteria.Nontuberculosis Mycobacteria distribution of 10 species,of which the major species were intracellular Mycobacterium(25.58%),Mycobacterium avium(20.93%)and Mycobacterium abscessus(18.60%),and 7 kinds of non tuberculosis Mycobacteria had not been detected.Conclusion Mycobacterium species widely distributed in Longyan City,Mycobacterial infection is mainly cased by Mycobacterium tuberculosis,nontuberculosis Mycobacteria infection in Longyan City is mainly caused by intracellular Mycobacterium,Mycobacterium avium,Mycobacterium abscessus.Identification of Mycobacterium species has a good guiding significance for the diagnosis and treatment of tuberculosis and nontuberculosis Mycobacterial disease.

关 键 词：分枝杆菌 菌种鉴定 基因芯片技术 结核病 

分 类 号：R446.5[医药卫生—诊断学]