HIV感染者NKG2D两种剪接型含量变化的研究  

Changes in the expression of two splicing isoforms of NKG2D in HIV-infected patients

作　　者：朱晓雨 刘婷婷[1] 张子宁[1] 姜拥军[1] ZHU Xiaoyu;LIU Tingting;ZHANG Zining;JIANG Yongjun(NHC Key Laboratory of AIDS Immunology,China Medical University,National Clinical Research Center for Laboratory Medicine,Key Laboratory of AIDS Immunology of Liaoning Province,Department of Laboratory Medicine,the First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院检验科国家卫生健康委员会艾滋病免疫学重点实验室国家临床医学研究中心辽宁省艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国艾滋病性病》2020年第10期1036-1040,共5页Chinese Journal of Aids & STD

基　　金：国家“十二五”科技重大专项(2012ZX10001-006)。

摘　　要：目的探究艾滋病病毒(HIV)感染者免疫细胞中NKG2D全长型(NKG2DFL)和截短型(NKG2DTR)两种剪接型含量及其与HIV感染疾病进展的关系,寻找影响NKG2D剪接型含量的可能因素。方法利用实时荧光定量聚合酶链反应(PCR)技术,分别检测HIV慢性感染者与抗病毒治疗后感染者及正常人的不同免疫细胞中NKG2D剪接型含量。在体外用TGF-β刺激后再检测外周血单个核细胞(PBMC)中的NKG2D剪接型含量。结果HIV慢性感染者PBMC中NKG2DFL、NKG2DTR的含量均低于抗病毒治疗后患者(P<0.01)及健康对照(P<0.01),且其NKG2DFL含量与CD4+T淋巴细胞(简称CD4细胞)数目呈明显负相关(r=-0.8405,P<0.01);CD8+T淋巴细胞(简称CD8细胞)和NK细胞中NKG2DFL、NKG2DTR的含量变化与PBMC一致,即HIV慢性感染者中含量低于正常对照(P<0.05),但CD4细胞中NKG2DFL、NKG2DTR含量在HIV慢性感染者反而高于正常对照(P<0.05);经TGF-β刺激后,PBMC中NKG2DFL、NKG2DTR含量均降低。结论HIV慢性感染者中NKG2DFL、NKG2DTR含量均较正常对照降低,尤其是在CD8、NK细胞中;TGF-β使PBMC中NKG2D两种剪接型含量下降。Objective To investigate the expression level of full-length(NKG2DFL)and truncated(NKG2DTR)NKG2D splicing isoforms in immune cells of HIV-infected patients and their relationship with HIV disease progression,so as to find possible factors affecting the splicing isoforms of NKG2D.Methods Real-time quantitative PCR was used to detect the NK-G2D splicing isoforms in different immune cells of the chronically HIV-infected patients,patients treated with antiretroviral therapy and normal controls.The NKG2D splicing isoforms in PBMC were detected after stimulation with TGF-βin vitro.Results The levels of NKG2DFL and NKG2DTR in PBMC of the chronically HIV-infected patients were lower than those of the patients treated with antiretroviral therapy(P<0.01)and normal controls(P<0.01),and NKG2DFL level was significantly negatively correlated with CD4 T cell counts of chronically HIV-infected patients(r=-0.8405,P<0.01).The level changes of NKG2DFL and NKG2DTR in CD8 T cells and NK cells were consistent with PBMC,i.e.the levels of NKG2DFLand NKG2DTR in the chronically HIV-infected patients were lower than those of normal controls(P<0.05),but the levels of NKG2DFL and NKG2DTR in CD4 T cells of the chronically HIV-infected patients were instead higher than those of normal controls(P<0.05).The levels of NKG2DFL and NKG2DTRin PBMC decreased after TGF-βstimulation.Conclusion The levels of NKG2DFL and NKG2DTR in the chronically HIV-infected patients are lower than those of normal controls,especially in CD8 T and NK cells.TGF-βreduces the levels of NKG2D two splicing isoforms in PBMC.

关 键 词：NKG2D 艾滋病病毒 可变剪接 实时荧光定量聚合酶链反应 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]