N-乙酰半胱氨酸对藏山羊母羊被公羊诱导发情效果与妊娠前期外周血液雌二醇和孕酮浓度的影响  

Effects of NAC on Induction of Estrus Synchronization in 2-year-old Ewes by Ram and Levels of Peripheral Blood Estradiol and Progesterone of Tibetan Goats During Pre Pregnancy

作　　者：宋天增[1] 巴贵[1] 次仁德吉[1] 德吉[1] 索朗达[1] 吴玉江[1] SONG Tian-zeng;Bagui;Cirendeji;Deji;Suolangda;WU Yu-jiang(Institute of Animal Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850009,China)

机构地区：[1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009

出　　处：《西藏农业科技》2020年第3期1-4,共4页Tibet Journal of Agricultural Sciences

基　　金：西藏自治区农业重大专项(XZ201901NA02)。

摘　　要：本研究旨在通过日粮中添加N-乙酰半胱氨酸,研究N-乙酰半胱氨酸对藏山羊公羊诱导同期发情率与妊娠前期血清雌二醇(E2)和孕酮(P4)浓度的影响。①2岁龄120只体重相近的经产藏山羊母羊,随机分组,空白对照组日粮中不添加NAC、试验Ⅰ组日粮中添加0.03%的NAC、试验Ⅱ组日粮中添加0.05%的NAC、试验Ⅲ组日粮中添加0.07%的NAC,1周后每日早晨6:00-6:30试情公羊戴试情布进入各组的母羊圈诱导发情、试情,统计各组5日内的发情母羊数量;②2岁龄120只体重相近的经产藏山羊母羊,随机分为空白对照组、试验Ⅰ、Ⅱ和Ⅲ组,各组每日配种的母羊单独小圈饲养1个月。对照组日粮中不添加NAC,试验Ⅰ组日粮中添加0.03%的NAC,试验Ⅱ组日粮中添加0.05%的NAC,试验Ⅲ组日粮中添加0.07%的NAC。测定试验第15日和30日外周血液E 2和P 4浓度。结果表明:①日粮中添加NAC,对藏山羊母羊被公羊诱导发情效果显著提升(P<0.01),以日粮中添加0.05%的NAC为最优,5日内公羊诱导同期发情率达100%;②日粮中添加NAC,提高藏山羊母羊妊娠期第15和30日外周血液E 2和P 4浓度,以日粮中添加0.05%的NAC为最高。本研究初步探究了NAC对藏山羊母羊被公羊诱导发情效果与妊娠前期外周血液雌二醇和孕酮浓度的影响,初步明确了妊娠前期饲粮中添加0.05%比例的NAC能提高藏山羊母羊被公羊诱导发情效果与妊娠前期外周血液雌二醇和孕酮浓度,但其作用机制,还待深入研究。本研究结果为藏山羊高效繁育提供理论上的借鉴。The purpose of this study was to study the effect of N-acetylcysteine on the estrus rate and the concentrations of estradiol(E2)and progesterone(P4)in the serum of Tibetan goats.(i)Two year old 120 ewes with similar body weight of Tibetan goats,No NAC was added to the blank control diet,0.03%NAC was added to the trial group Ⅰ diet,0.05%NAC was added to the trial group Ⅱ diet,0.07%NAC was added to the trial group Ⅲ diet after random grouping,one week later,every morning,from 6:00-6:30 in the morning,the ram wears the test cloth and enters into the ewe pen of each group to induce oestrus and test oestrus.Count the number of oestrus ewes in each group within 5 days.(ii)Two year old 120 ewes with similar body weight of Tibetan goats,they were randomly divided into three groups:Blank control group,experimental group Ⅰ,experimental group Ⅱ and experimental group Ⅲ.Ewes of each group were bred in separate small circles for one month every day.No NAC was added to the blank control diet,0.03%NAC was added to the trial group Ⅰ diet,0.05%NAC was added to the trial group Ⅱ diet,0.07%NAC was added to the trial group Ⅲ diet.The peripheral blood E2 and P4 concentrations were measured on the 15th and 30th day of the experiment.The results show that:(i)When NAC was added to the diet,the estrus induction effect of 2-year-old ewes was significantly improved(P<0.01),and 0.05%NAC in the diet was the best,and the estrus induction rate of ewes was 100%within 5 days;(ii)The concentration of E2 and P4 in the peripheral blood of 2-year-old ewes on the 15th and 30th day of pregnancy was increased by adding NAC in the diet,and the highest concentration was 0.05%NAC in the diet.In this study,the effects of NAC on the induction of estrus and the concentrations of estradiol and progesterone in the peripheral blood of 2-year-old ewes were studied.It was found that the 0.05%NAC in the pre pregnancy diet could improve the induction of estrus and the concentrations of estradiol and progesterone in the peripheral blood of 2-year-o

关 键 词：N-乙酰半胱氨酸 藏山羊 诱导发情 外周血液 雌二醇 孕酮 浓度 

分 类 号：S826[农业科学—畜牧学]