超临界CO2萃取法提取西藏红花杜鹃叶中化学成分及其GC-MS初步分析研究  

Supercritical Carbon Dioxide Extraction for Leaves of Rhododendronspanotrichum Balf.f.et W.W.Smith and Preliminary Analysis of Chemical Composition by Gas Chromatography Mass Spectrometry(GC-MS)

作　　者：拉巴次旦[1] 四郎玉珍[1] 班旦[1] 格桑卓嘎[1] 郝宝成[2] 吴金措姆[1] Labacidan;Silangyuzhen;Bandan;Gesangzhuoga;HAO Bao-cheng;Wujincuomu(Institute of Animal Science and Veterinary,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850000,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences,Gansu Lanzhou 730050,China)

机构地区：[1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《西藏农业科技》2020年第3期35-38,共4页Tibet Journal of Agricultural Sciences

基　　金：西藏自治区财政涉农推广类项目“藏兽药研发与示范”。

摘　　要：确定超临界CO2萃取西藏红花杜鹃叶的最优提取工艺,并初步鉴定分析其提取物的主要化学成分组成。以提取率为评价指标,利用正交优化法对开花期西藏红花杜鹃叶进行超临界CO2萃取,采用气相色谱-质谱联用技术(gas chromatgraphy-mass spectrometry,GC-MS)对提取物进行化学成分分析。超临界CO2萃取西藏红花杜鹃叶的最优工艺条件为将红花杜鹃叶粉碎为200目、萃取压力20 MPa、萃取温度为40℃、反应时间2.5 h,GC-MS检测分离出了23种含量较高的化合物。经与质谱谱图数据库比对,西藏红花杜鹃叶超临界CO2萃取提取物主要为烷烃(39.54%)、酯(26.94%)、烯(24.48%)、酰胺类(8.78%)等化合物。To determine the optimal extraction process of the leaves of Rhododendron spanotrichum Balf.f.et W.W.Smith were extracted with supercritical carbon dioxide and preliminarily identify and analyze the main chemical components of the extract.Taking the extraction rate as the evaluation index,the leaves of Rhododendron spanotrichum Balf.f.et W.W.Smith were extracted with supercritical carbon dioxide by orthogonal optimization method.The chemical components of the extract were analyzed by gas chromatography mass spectrometry(GC-MS).The optimum conditions for supercritical carbon dioxide extraction of the leaves of Rhododendron spanotrichum Balf.f.et W.W.Smith were as follows:Crushing into 200 mesh,extraction pressure 20 MPa,extraction temperature 40℃,reaction time 2.5 hours.23 compounds with high content were detected and separated by GC-MS.Compared with the mass spectrum database,supercritical carbon dioxide extraction for the leaves of Rhododendron spanotrichum Balf.f.et W.W.Smith was mainly composed of alkanes(39.54%),esters(26.94%),alkenes(24.48%)and amides(8.78%),etc..

关 键 词：红花杜鹃 超临界CO2萃取法 化学成分 GC-MS 

分 类 号：S685.14[农业科学—观赏园艺]