Penicillium sp.YJM-2013 induces ginsenosides biosynthesis in Panax ginseng adventitious roots by inducing plant resistance responses  

作　　者：Shi-hui Wang Wen-xia Liang Jun Lu Lu Yao Juan Wang Wen-yuan Gao 

机构地区：[1]Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China [2]Key Laboratory of Systems Bioengineering Ministry of Education,Tianjin University,Tianjin 300072,China [3]Tianjin Key Laboratory for Modem Drug Delivery and High Efficiency,School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China

出　　处：《Chinese Herbal Medicines》2020年第3期257-264,共8页中草药（英文版）

基　　金：The work was supported by National Natural Science Foundation of China NSFC(No.81703639).

摘　　要：Objective:Fusarium oxysporum is a common pathogenic fungus in ginseng cultivation.Both pathogens and antagonistic fungi have been reported to induce plant resistance responses,thereby promoting the accumulation of secondary metabolites.The purpose of this experiment is to compare the advantages of one of the two fungi,in order to screen out more effective elicitors.The mechanism of fungal elicitor-induced plant resistance response is supplemented.Methods:A gradient dilution and the dural culture were carried out to screen strains.The test strain was identified by morphology and 18 s rDNA.The effect of different concentrations(0,50,100,200,400 mg/L)ofPenicillium sp.YJM-2013 and F.oxysporum on fresh weight and ginsenosides accumulation were tested.Signal molecules transduction,expression of transcription factors and functional genes were investigated to study the induction mechanism of fungal elicitors.Results:Antagonistic fungi ofF.oxysporum was identified as Penicillium sp.YJM-2013,which reduced root biomass.The total ginsenosides content of Panax ginseng adventitious roots reached the maximum(48.95±0.97 mg/g)treated with Penicillium sp.YJM-2013 at 200 mg/L,higher than control by 2.59-fold,in which protopanoxadiol-type ginsenosides(PPD)were increased by 4.57 times.Moreover,Penicillium sp.YJM-2013 activated defense signaling molecules,up-regulated the expression of PgWRKY 1,2,3,5,7,9 and functional genes in ginsenosides synthesis.Conclusion:Compared with the pathogenic fungi F.oxysporum,antagonistic fungi Penicillium sp.YJM-2013 was more conducive to the accumulation of ginsenosides in P.ginseng adventitious roots.Penicillium sp.YJM-2013 promoted the accumulation of ginsenosides by intensifying the generation of signal molecules,activating the expression of transcription factors and functional genes.

关 键 词：antagonistic fungi functional genes fungi elicitors ginsenosides biosynthesis HPLC-ESI-MS^n Panax ginseng C.A.Meyer signal molecules transcription factors 

分 类 号：S567.51[农业科学—中草药栽培]