Comparative transcriptome and co-expression analysis reveal key genes involved in leaf margin serration in Perilla frutescens  

作　　者：Qi Shen Dong Zhang Tian-yuan Zhang Yang-yang Xu De-gang Zhao 

机构地区：[1]Institute of Medical Plant Physiology and Ecology,School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China [2]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]Guizhou Academy of Agricultural Sciences,Guiyang 550008,China [4]China Center for Information Industry Development,Beijing 100036,China

出　　处：《Chinese Herbal Medicines》2020年第3期265-272,共8页中草药（英文版）

基　　金：This work was funded by National Natural Science Foundation for regional fund(31860391);Guizhou Province Science and Technology Plan Project(Qian science platform[2019]5656);National Major Project for Breeding New Transgenic Varieties(2016ZX08010-003).

摘　　要：Objective:In this study,we aimed to identify the genes involved in leaf margin serration in Perilla frutescens.P.frutescens(Family:Lamiaceae)is widely grown in Asian countries.Perilla leaf is the medicinal part stipulated in the Chinese Pharmacopoeia.There are mainly two types of perilla leaves:one with serrated leaf margin which is the phenotype described in the pharmacopoeia and the other with smooth leaf margin.Methods:Transcriptome sequencing,co-expression analysis,and qRT-PCR analysis of six perilla tissues sampled from two different phenotypes(serrated and smooth leaves)were performed.Results:Forty-three differentially expressed genes(DEGs),which may potentially regulate leaf shape,were identified through de novo transcriptome sequencing between the two groups.Genes involved in leaf shape regulation were identified.Simultaneously,we validated five DEGs by qRT-PCR,and the results were consistent with the transcriptome data.In addition,1186 transcription factors(TFs)belonging to 45 TF families were identified.Moreover,the co-expression network of DEGs was constructed.Conclusion:The study identified the key genes that control leaf shape by comparing the transcriptomes.Our findings also provide basic data for further exploring P.frutescens,which can help study the mechanism of leaf shape development and molecular breeding.

关 键 词：leaf shape development Perilla frutescens(L.)Britt. transcriptome sequencingw rinkly leaves 

分 类 号：S567.219[农业科学—中草药栽培]