红耳龟植物血凝素反应峰值与血液指标的关系及温度对其反应模式的影响  

Relationship Between Maximal Phytohemagglutinin-P Response Value and Hematological Indices,and Effects of Temperatures on the Response Patterns of Trachemys scripta elegans

作　　者：吴文欣 吴海燕 杨雨晴 冯文红 许洋溢 刁迎珠 汪寅 张志强[1] WU Wenxin;WU Haiyan;YANG Yuqing;FENG Wenhong;XU Yangyi;DIAO Yingzhu;WANG Yin;ZHANG Zhiqiang(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《四川动物》2020年第6期639-645,共7页Sichuan Journal of Zoology

基　　金：国家自然科学基金项目(31170379);安徽省舒城县“三区”科技人才项目(2020);安徽农业大学2019年省级大学生创新创业训练计划项目创新训练项目(201910364042);安徽农业大学2020年省级质量工程项目教学改革研究重点项目(062)。

摘　　要：为探究红耳龟Trachemys scripta elegans对植物血凝素(PHA-P)的反应峰值及其与血液指标的关系,以及温度对其反应模式的影响,测定了22℃、最适注射浓度条件下注射前和到达峰值时,血液中各型白细胞的百分比和血浆杀菌能力,以及经20℃、24℃、28℃和32℃处理6周后,红耳龟对PHA-P最适注射浓度的反应模式。结果显示,22℃下经1.0 g·L^-1、2.5 g·L^-1和5.0 g·L^-1浓度PHA-P处理后,分别在注射后24 h、48 h和24 h到达反应峰值。选2.5 g·L^-1为最适注射浓度,达峰值时淋巴细胞的百分比显著升高(P<0.05),其他类型白细胞的百分比、嗜中性粒细胞与淋巴细胞的比值及血浆杀菌能力注射前后的差异均无统计学意义(P>0.05);20℃、24℃、28℃和32℃组分别处理6周后,红耳龟对PHA-P的反应峰值见于48 h、24 h、24 h和24 h,峰值间的差异无统计学意义(P>0.05);注射后24~48 h,20℃组的反应值升高,32℃组下降,24℃和28℃组保持相对稳定,但差异均无统计学意义(P>0.05)。红耳龟对PHA-P的反应峰值与淋巴细胞数量增加有关,在其适宜生存的温度范围内随温度升高而前移,可以通过预先激活适应性免疫能力来提高红耳龟对高温的适应能力。To explore the relationship between maximal phytohemagglutinin-P(PHA-P)response value and hematological indices in the red-eared sliders(Trachemys scripta elegans),and the effects of temperatures on their PHA-P response patterns,variations of different subtypes of leukocyte and plasma bactericidal capacity from blood prior to injection and the maximal PHA-P response value were measured with optimal PHA-P injection concentration at 22℃,and the PHA-P response patterns to optimal PHA-P injection concentration were compared after 6 weeks acclimation of 20℃,24℃,28℃,and 32℃.Under the conditions of 22℃water temperature,T.scripta elegans was treated with 1.0 g·L^-1,2.5 g·L^-1,and 5.0 g·L^-1concentration of PHA-P solutions,the responsiveness to PHA-P peaked at 24 h,48 h,and 24 h after injection,respectively,and the concentration of 2.5 g·L^-1was selected as the optimal,and the percentage of lymphocytes was significantly increased(P<0.05),but no significant differences were found for other subtypes of leukocyte,ratio of neutrophils to lymphocytes,and plasma bactericidal capacity from circulation(P>0.05)while reaching the peak.After the turtles were treated with different water temperatures of 20℃,24℃,28℃,and 32℃for 6 weeks,they were injected with 2.5 g·L^-1PHA-P concentration,respectively.The responsiveness to PHA-P peaked at 48 h,24 h,24 h,and 24 h for 20℃,24℃,28℃and 32℃group,respectively,and no significant differences were found for the maximal PHA-P values among different temperature groups(P>0.05).From 24 h to 48 h postinjection,the responsiveness to PHA-P was increased at 20℃group,decreased at 32℃group,and remained relatively stable at 24℃and 28℃group,but no significant differences were found.In T.scripta elegans,the responsiveness to PHA-P solution is related to the increased number of lymphocytes,and shifts forward with arising temperatures within moderate temperature ranges for their survivals,these variations may in advance activate adaptive immunity to enhance their adapta

关 键 词：红耳龟 温度 植物血凝素 淋巴细胞 免疫生态学 

分 类 号：Q959.6[生物学—动物学] Q958.1