柿属新种德阳柿组培快繁技术研究  

Study on Tissue Culture and Rapid Propagation of Persimmon from Deyang,a New Species of Persimmon

作　　者：王洋 邱晓[1,2] 孙海莲[1,2] 常虹 徐莉清[3] WANG Yang;QIU Xiao;SUN Hai-lian(Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot,Inner Mongolia 010031;Inner Mongolia Prataculture Research Center,Chinese Academy of Sciences,Hohhot,Inner Mongolia 010031;Huazhong Agricultural University,Wuhan,Hubei 430070)

机构地区：[1]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031 [2]中国科学院内蒙古草业研究中心,内蒙古呼和浩特010031 [3]华中农业大学,湖北武汉430070

出　　处：《安徽农业科学》2020年第22期51-55,共5页Journal of Anhui Agricultural Sciences

摘　　要：为了使德阳柿能够通过组织培养的方式在短期内快速繁殖,探究德阳柿培养基的最适培养条件。取一年生德阳柿枝条上的休眠芽茎段无菌处理后作为外植体,选择不同的基本培养基,对德阳柿组培快繁技术进行研究。结果表明,以MS为基本培养基的德阳柿休眠芽组培苗萌发率达81.13%,以MS(1/2N)为基本培养基的德阳柿休眠芽萌发率为78.05%;德阳柿诱导休眠芽以MS(1/2N)为基本培养基的长势要优于以MS为基本培养基。由此可知,MS(1/2N)培养基是德阳柿幼芽初代培养的最适培养基。该研究提供了德阳柿初代培养的最适培养基,为其短期内大量繁殖提供了技术指导和理论支持。In order to make Deyang persimmon multiply rapidly in a short time by tissue culture,the optimum culture conditions of Deyang persimmon were studied.The stem segment of dormant buds on the branches of deyang persimmon was treated asepsis and used as explants.The germination rate of the dormant buds in Deyang persimmon using MS as the basic medium was 81.13%,while the germination rate of the dormant buds in Deyang persimmon using MS(1/2 n)as the basic medium was 78.05%.The growth rate of the dormant buds in Deyang persimmon with MS(1/2 n)as the basic medium was better than that with MS as the basic medium.Finally,it can be concluded that MS(1/2 n)medium was the optimal medium for the primary bud culture of deyang persimmon.This study provided the optimum medium for the primary culture of Deyang persimmon and provided technical guidance and theoretical support for its short-term mass reproduction.

关 键 词：德阳柿 组培快繁 初代培养 继代培养 无菌操作 接种 育苗 

分 类 号：S665.2[农业科学—果树学]