不同条件下牙鲆血清免疫学指标的比较  

Comparison of Serum Immune Indices of Paralichthys olivaceus under Different Conditions

作　　者：宋立民 李明泽 李仰真[2] 马林[1] 吴会民[1] 刘义[1] 李楠[1] SONG Li-min;LI Ming-ze;LI Yang-zhen(Tianjin Fishery Research Institute,Tianjin 300221;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences(CAFS),Qingdao,Shandong 266071)

机构地区：[1]天津市水产研究所,天津300221 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《安徽农业科学》2020年第22期94-98,共5页Journal of Anhui Agricultural Sciences

基　　金：天津市农业发展服务中心青年科技创新项目(ZXKJ201908,ZXKJ201911)。

摘　　要：为评价遗传背景、体重、黑化、运输应激等对牙鲆(Paralichthys olivaceus)血清免疫学指标的影响,以引进的“鲆优2号”抗病牙鲆和普通牙鲆为试验材料,通过肉眼区分无眼侧有无黑化,在不同生长阶段采集样本,采样前有无施行包装运输等手段,将研究对象分为7个试验组,分别测定溶菌酶(LYS)活力、酸性磷酸酶(ACP)活力、超氧化物歧化酶(SOD)活力、免疫球蛋白M(IgM)含量、补体3(C3)和补体4(C4)含量等6种免疫学指标。结果表明:试验组1各项检测指标均低于试验组2,但差异均不显著(P>0.05);试验组4各项检测指标均低于试验组7,其中LYS、ACP、SOD活力及IgM含量差异极显著(P<0.01),C 4含量差异显著(P<0.05),C3含量差异不显著(P>0.05);试验组1各检测指标均低于试验组7,且差异超级显著(P<0.001);试验组2各检测指标均小于试验组4,SOD活力及IgM、C3含量差异超级显著(P<0.001),LYS活力差异极显著(P<0.01),C4含量差异显著(P<0.05),ACP活力无显著差异;试验组3与试验组4各指标差异均不显著(P>0.05);比较试验组5、试验组6与试验组7的检测结果发现,只有试验组5中C3含量极显著低于试验组6(P<0.01),其余指标差异均不显著。通过一定的育种手段可使养殖对象在某一个或多个方面获得可遗传的免疫优势;随着牙鲆个体的生长,体重增加,抗病力逐渐增强;牙鲆的抗病能力与黑化与否的相关性未达到显著水平;对于同一遗传背景,经历运输(未产生休克)与未经历运输样本鱼的6种免疫学指标差异不显著。In order to evaluate the influence of genetic background,body weight,hypermelanosis and transportation stress on the serum immune indices of Paralichthys olivaceus,the subjects were divided into 7 experimental groups,according to blind-side with hypermelanosis or not,different growth stages,and sampling with transportation stress or not,from the resistant flounder and common flounder which were used as experimental materials.Lysozyme(LYS)activity,acid phosphatase(ACP)activity,superoxide dismutase(SOD)activity,immunoglobulin M(IgM)content,complement C3 content,C4 content were measured and compared separately of the above groups.The results indicated that the test items of experimental group 1 were less than those of group 2,but the difference was not significant(P>0.05).The test items of experimental group 4 were lower than those of group 7,and there were highly significant differences(P<0.01)in LYS activity,ACP activity,SOD activity and IgM content,while the difference in C4 content was significant(P<0.05),there was no significant difference(P>0.05)in C3 content.The test items of experimental group 1 were lower than those of group 7,and the difference were super significant(P<0.001).The test items of experimental group 2 were lower than those of group 4,and there were super significant differences(P<0.001)in SOD activity,IgM content and C3 content,while highly significant difference(P<0.01)in LYS activity,significant difference(P<0.05)in C4 content,no significant difference(P>0.05)in ACP activity.There were no significant difference(P>0.05)in 6 immune indices between experimental group 3 and group 4.Only C3 content of group 5 was highly significant(P<0.01)lower than that of group 6,by comparing the test items of experimental group 5,group 6 and group 7.The results showed that heritable immune advantage in one or more aspects could be obtained by cultured animals,according to certain breeding methods.The disease resistance was gradually increased with the growth of Paralichthys olivaceus individuals.There was no s

关 键 词：牙鲆 遗传背景 体重 黑化 运输应激 免疫学指标 

分 类 号：S917.4[农业科学—水产科学]