不同品种山银花花期有效成分含量研究  

Study on the Contents of Active Components of Different Varieties of Lonicera japonica Thunb.in the Flowering Stage

作　　者：石前 庾韦花[1] 蒙平[1] 张向军[1] 潘颖南[1] 樊永生 SHI Qian;YU Wei-hua;MENG Ping(Institute of Biotechnology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007)

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西忻城县农业技术推广中心,广西来宾546200

出　　处：《安徽农业科学》2020年第22期170-171,201,共3页Journal of Anhui Agricultural Sciences

基　　金：广西农业科学院基本科研业务专项(桂农科2020YM64);广西忻城金银花试验站项目(桂TS201428);广西农业科学院优势学科团队项目(2015YT56)。

摘　　要：[目的]对4个广西本地山银花药材的质量及从河南引种的1个金银花品种(对照)进行品质评价,研究不同品种山银花不同采收期与有效成分含量的关系,为广西山银花资源合理开发和综合利用提供依据。[方法]采收4个品种山银花白蕾期、银花期、金花期的花器官进行处理,采用高效液相色谱法,分别测定其木犀草苷、绿原酸、氨基酸总量等有效成分含量。[结果]对比4个品种山银花不同花期的有效成分含量,其中木犀草苷含量和氨基酸总量最高的是红-RD,分别达0.023%和13.000%,但低于对照品种;木犀草苷含量最低的是HX-RD,仅为0.001%;氨基酸总量最低的是MH-RD,为8.240%;绿原酸含量最高的是HN-RD,达6.587%;最低的是MH-RD,仅为2.400%;不同品种之间的有效成分含量呈现显著差异。从不同采收期来看,白蕾期的氨基酸含量均最高,木犀草苷含量不同采收期差异不显著,绿原酸不同采收期含量差异水平不一。[结论]作为广西乡土品种,红-RD和HN-RD的有效成分含量高,有很好的培育价值;对照品种H-RD(河南引进)是优良的金银花品种,适合在广西栽培;山银花最佳采收期为白蕾期,其有效成分含量最高,品质最好。[Objective]To study the relationship between different harvesting periods and the content of effective components of different varieties of Lonicera japonica by evaluate the quality of four Guangxi Lonicera japonica and one Lonicera japonica(CK)introduced from Henan,so as to provide the basis for the rational development and comprehensive utilization of Guangxi Lonicera japonica resources.[Method]The flower organs of four varieties were collected and treated in the different growing stage,HPLC was used to determine the contents of galuteolin,chlorogenic acid and total amino acid.[Results]By comparing the contents of active components in the four varieties of Lonicera japonica at different flowering stages,red-RD had the highest contents of galuteolin and the total amount of amino acid,but lower than the CK.The lowest contents of galuteolin was HX-RD of 0.001%,the lowest contents of amino acid was MH-RD of 8.240%,the highest contents of chlorogenic acid was HN-RD which up to 6.587%,the lowest was MH-RD which just at 2.400%.The contents of active components were significantly different among different varieties.From the perspective of different harvesting periods,the content of amino acids in the white bud stage was the highest,the content of luteolin was not significantly different at different harvesting stages,and the level of difference in the content of chlorogenic acid was different at different harvesting stages.[Conclusion]As native varieties in Guangxi,red-RD and HN-RD have high content of effective components and good breeding value.The H-RD(introduced from henan province)is suitable for cultivation in Guangxi.White bud period was the best harvest period for Lonicera japonica which has the highest content of active components and the best quality.

关 键 词：山银花 花期 采收期 有效成分 含量测定 

分 类 号：R284[医药卫生—中药学]