浙江省供精者精液分析参考区间的建立  

Establishment of biological reference interval of semen parameters in Zhejiang sperm donors

作　　者：吴颖 宋银莉 沈晓芳 WU Ying;SONG Yinli;SHEN Xiaofang(Zhejiang Maternal Child and Reproductive Health Center,Hangzhou 310012,Zhejiang,China)

机构地区：[1]浙江省妇幼和生殖保健中心,浙江杭州310012

出　　处：《检验医学》2020年第11期1094-1098,共5页Laboratory Medicine

摘　　要：目的建立浙江省供精者精液分析参考区间,为临床患者生育力评估提供科学实验室判断依据。方法采用世界卫生组织(WHO)第5版《人类精液检查与处理实验室手册》(简称WHO第5版)分析法对2017年1月—2019年8月浙江省人类精子库4122名初筛健康供精志愿者进行精液常规分析和精子形态学分析,检查精液pH值和体积、精子浓度和总数、前向运动精子百分比、精子正常形态率等参数,确定精液分析参考区间,且与WHO第5版参考区间进行比较。结果浙江省供精者精液分析参考区间为:精液体积≥1.8 mL,精子总数≥51×10^6/次射精,精子浓度≥16×10^6/mL,PR精子百分比≥20%,精子正常形态率≥5%,精液pH值≥8.0。结论浙江省供精者精液分析参考区间与WHO第5版相比,PR精子百分比明显偏低,其余各项参考值也略有不同,而从精液各参数数值分布看,健康男性各项精液参数分布与WHO第5版数据范围都有一定差异,高百分位数上(P50以上)明显偏低。随男性年龄的增加,特别是40岁以上,精液质量明显下降。Objective To investigate the reference interval of semen parameters from sperm donors in Zhejiang,and to provide a reference for fertility evaluation of clinical patients.Methods Semen samples from 4122 sperm donors at Zhejiang Human Sperm Bank were collected from Januray 2017 to August 2019.Routine semen analysis and semen morphology analysis were performed according to the World Health Organization(WHO)the 5th-edition guidelines,and the pH value,volume,sperm concentration,total sperm count,sperm progressive motility and the rate of sperm normal morphology were determined.The reference interval of semen parameters was obtained,which was compared with that of WHO the 5th-edition guidelines.Results The reference intervals of semen parameters were as follows,volume≥1.8 mL,total sperm count≥51×10^6/time,sperm concentration≥16×10^6/mL,PR≥20%,the rate of sperm normal morphology≥5%and the pH value≥8.0.Conclusions Compared with the reference interval of WHO the 5th-edition guidelines,Zhejiang sperm donors has low PR,and the reference intervals of the other semen parameters were different.The numerical distribution of semen parameters in Zhejiang is slightly different from that of WHO the 5th-edition guidelines,especially in the high percentile above P50.With the increase of sperm donors,especially for males over 40 years old,sperm quality decreases significantly.

关 键 词：参考区间 精液分析 供精者 

分 类 号：R446.1[医药卫生—诊断学]