Characterization of Bacterial Community,Ammonia-Oxidizing Bacteria,and Nitrospira During the Operation of a Commercial-Scale Recirculating Aquaculture System for Culturing Pufferfish Takifugu rubripes  

作　　者：MA Yuexin YU Zichao DU Xin ZHANG Tao WANG Ning TAO Wei 

机构地区：[1]Key Laboratory of Environment Controlled Aquaculture,Ministry of Education,Dalian 116023,China [2]Liaoning Key Laboratory of Marine Animal Immunology and Disease Control,Dalian Ocean University,Dalian 116023,China [3]Dalian Tianzheng Industry Company Limited,Dalian 116011,China [4]College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China

出　　处：《Journal of Ocean University of China》2020年第6期1399-1408,共10页中国海洋大学学报（英文版）

基　　金：This research was supported by the National Key R&D Program of China(No.2017YFD0701700);the National Natural Science Foundation of China(Nos.31472312 and 31672673).

摘　　要：We investigated the changes in communities of bacteria,ammonia-oxidizing bacteria,and Nitrospira during the operation of a pufferfish Takifugu rubripes recirculating aquaculture system by using high-throughput DNA sequencing.Differences in bacterial communities were observed at days 1-32,47-62 and 78-93 of biofilm development by using 16S rRNA gene pyrosequencing.The relative abundance of Proteobacteria(Gammaproteobacteria)increased,while that of Bacteroidetes(Flavobacteria)decreased.The proportions of Nitrosomonas and Nitrospina ranged from 0.02%to 0.30%and from 0.02%to 0.83%,respectively.Ammonia monooxygenase gene pyrosequencing revealed that the top three operational taxonomic units were related to Nitrosomonas aestuarii(17.5%-61.1%),uncultured beta proteobacterium clone B67S-54(1.9%-45.2%),and uncultured bacterium clone AZPa8(3.6%-24.7%).Nitrite oxidoreductase gene pyrosequencing revealed that the relative abundance of the dominant strain Nitrospira sp.Ecomares 2.1 increased,but that of the abundant species Nitrospira marina decreased.Our results demonstrated that the communities of bacteria,ammonia-oxidizing bacteria,and Nitrospira were changing during the operation of the pufferfish recirculating aquaculture system.

关 键 词：BIOFILTER bacterial community ammonia-oxidizing bacteria NITROSPIRA recirculating aquaculture system 

分 类 号：S917.1[农业科学—水产科学]