Seasonal Variations of Picoeukaryote Community Structure in Zhoushan Fishing Ground,East China Sea  

作　　者：YANG Mingyu LIU Qiang MI Chunrong ZHAO Shujiang 

机构地区：[1]National Engineering Research Center of Marine Facilities Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China [2]Chinese Research Academy of Environmental Sciences,Institute of Water Environment Science,Beijing 100020,China [3]Institute of Zoology,Chinese Academy of Sciences,Beijing 100000,China

出　　处：《Journal of Ocean University of China》2020年第6期1471-1479,共9页中国海洋大学学报（英文版）

基　　金：We thank the captain and all crews of the RV‘Zhehaiyan 2’for their assistance in sample collection.This work was supported by the Nonprofit Research Project of the State Oceanic Administration(Nos.201505025 and 201505003).

摘　　要：Picoeukaryotes(<2-3μm)are major oceanic primary producers and play a crucial role for the functioning of marine ecosystems.However,the community structure of such communities remains poorly understood,especially in the East China Sea(ECS).We investigated the seasonal variations of abundance and diversity of picoeukaryotes,and recorded environmental variables,along a transect in Zhoushan Fishing Ground.High-throughput sequencing was used for sequencing the V4-V5 variable region within the 18S rRNA gene to analyze genetic diversity and relative abundance of picoeukaryotes.A total of 40 phyla,68 classes,99 orders,126 families,and 140 genera were observed.The estimated richness and diversity indices were both higher in each season.The composition and dominant picoeukaryotes changed significantly at the class level with the seasons alternating.Basidiomycota,Ciliophora,Ascomycota and Cryptomonadales were observed throughout the year.The Linear Discriminant Analysis(LDA)revealed the predominant discriminant taxa of four seasonal groups.Redundancy analysis(RDA)showed that NO2^−and salinity played very important roles to picoeukaryotes for all the spring samples and DS5 sample in winter,PO4^3−and pH affected mostly picoeukaryotes for winter samples and autumn samples.

关 键 词：PICOEUKARYOTES Zhoushan Fishing Ground high-throughput sequencing 18S rRNA gene community structure DIVERSITY 

分 类 号：Q958[生物学—动物学]