顺义区2018年腹泻病例粪便中致泻大肠埃希氏菌耐药监测结果分析  

Analysis of drug resistance of diarrheal Escherichia coli in stool of diarrhea cases in Shunyi Diarrheal in 2018

作　　者：杨杰[1,2] 李文 冀国强[1] 马红梅[1,2] 张爽[1,2] 张赫 李颖[1,2] YANG Jie;LI Wen;JI Guoqiang;MA Hongmei;ZHANG Shuang;ZHANG He;LI Ying(Shunyi District Center for Disease Control and Prevention,Beijing,China,101300)

机构地区：[1]北京市顺义区疾病预防控制中心,北京101300 [2]北京市顺义区疾病预防控制中心微生物感染性疾病检测工作站

出　　处：《安徽预防医学杂志》2020年第5期386-389,共4页Anhui Journal of Preventive Medicine

摘　　要：目的了解北京顺义地区2018年腹泻病例粪便标本中分离的致泻大肠埃希氏菌(Diarrheagenic Escherichia coli,DEC)耐药状况。方法:收集北京顺义区2018年腹泻病例粪便标本,分离并鉴定5种DEC,使用肉汤稀释法测定DEC对20种抗菌药的耐药率、多重耐药率和耐药谱。结果:腹泻病例粪便中DEC检出率10.19%(37/363),其中产毒性大肠埃希氏菌构成比最高,为59.46%(22/37)。DEC耐药率较高的抗菌药包括氨苄西林(62.16%)、萘啶酸(59.46%)、复方新诺明(35.14%)、磺胺异噁唑(32.43%)、四环素(24.32%),对氨苄西林-舒巴坦、阿莫西林-克拉维酸的中介率分别达到48.65%和24.32%。DEC优势耐药谱为萘啶酸+氨苄西林,多重耐药率为24.32%(9/37)。结论腹泻病例中DEC耐药形势较为严峻,左氧氟沙星、环丙沙星仍然是临床治疗DEC感染的首选药物。Objective To study the antibiotic resistance characteristics of Diarrheagenic Escherichia coli(DEC)isolated from diarrheal patients in Shunyi,Beijing.Methods Fecal samples of diarrhea cases in Shunyi District of Beijing in 2018 were collected,and five kinds of DEC were isolated and identified.Results The prevalence of DEC in diarrhea patients was 10.19%(37/363),and the dominate species was enterotoxigenicE.Coli with the constituent ratio value 59.46%(22/37).The drug resistance rate of DEC from high to low were Ampicillin(62.16%),Naphthoic acid(59.46%),Sulfamethoxazole Tablets(35.14%),Sulfamidoxazole(32.43%),Tetracycline(24.32).Intermediate drug resistance rate of Ampicillin/Sulbactam and Amoxicillin/Clavulanic acid were 48.65%and 24.32%,respectively.The dominant drug resistance spectrum of DEC was NAL+AMP.The antibiotic susceptibility tests indicated that 24.32%(9/37)of the isolates exhibited resistance to three or more types of antibiotic.Conclusion Drug resistance situation of DEC isolated in diarrheal patients is more serious.Levofloxacin and Ciprofloxacin are still the first choice in the treatment of DEC infection.

关 键 词：致泻大肠埃希菌 耐药 腹泻 

分 类 号：R378[医药卫生—病原生物学]