雷诺嗪联合血栓通胶囊对糖尿病大鼠心肌缺血再灌注损伤过程中细胞自噬及心肌核因子-κB、人磷酸化Akt蛋白、IP7水平的影响  

Effects of Ranolazine Combined with Xueshuantong Capsule on Cell Autophagy and Myocardial NF-κB, p-Akt, IP7 Levels During Myocardial Ischemia-reperfusion Injury in Diabetic Rats

作　　者：杜汉承 DU Han-Cheng(Department of Basic Medicine,Quanzhou Medical College,Quanzhou 362011,China)

机构地区：[1]泉州医学高等专科学校基础医学部,福建泉州362011

出　　处：《中国药物经济学》2020年第9期39-43,共5页China Journal of Pharmaceutical Economics

摘　　要：目的探讨雷诺嗪联合血栓通胶囊对糖尿病大鼠心肌缺血再灌注损伤(MIRI)过程中细胞自噬及心肌核因子-κB(NF-κB)、人磷酸化Akt蛋白(p-Akt)、IP7水平的影响。方法随机将32只SD雄性大鼠分为假手术组、缺血再灌注组、雷诺嗪组、雷诺嗪联合血栓通胶囊组。观察各组心率、收缩压、舒张压、左室收缩压(LVSP)、左室舒张压(LVDP)、左室内压最大上升速率(+dp/dtmax)、左室内压最大下降速率(-dp/dtmax);心肌梗死面积、自噬水平及心肌IP7、p-核因子-κB、p-Akt水平。结果缺血再灌注组、雷诺嗪组、雷诺嗪联合血栓通组的LVSP、LVDP、+dp/dtmax和-dp/dtmax显著低于假手术组(P<0.05);与缺血再灌注组比较,雷诺嗪组和雷诺嗪联合血栓通组的LVSP、LVDP、+dp/dtmax和-dp/dtmax显著增加,且雷诺嗪联合血栓通组显著高于雷诺嗪组,差异有统计学意义(P<0.05);3组SD大鼠的心肌缺血程度比较差异无统计学意义(P>0.05);与缺血再灌注组比较,雷诺嗪组和雷诺嗪联合血栓通组心肌梗死程度显著下降,且雷诺嗪联合血栓通组显著低于雷诺嗪组,差异有统计学意义(P<0.05);与缺血再灌注组比较,雷诺嗪组和雷诺嗪联合血栓通胶囊组Beclin-1蛋白和LC3-Ⅱ蛋白表达量显著下降(P<0.05),且雷诺嗪联合血栓通组改善程度优于缺血再灌注组(P<0.05);与缺血再灌注组比较,雷诺嗪组和雷诺嗪联合血栓通胶囊组p-Akt表达显著增加(P<0.05),IP7和p-核因子-κB表达量显著下降(P<0.05),且雷诺嗪联合血栓通组改善程度优于缺血再灌注组(P<0.05)。结论雷诺嗪联合血栓通胶囊能够有效保护心肌的舒缩功能,降低心肌梗死面积,抑制心肌细胞过度自噬,通过调节降低IP7水平,恢复Akt活性,降低核因子-κB水平,从而保护缺血再灌注引起的细胞损伤。Objective To explore the effect of renolazine combined with Xuesentong Capsule on cell autophagy and the levels of NF-B, p-Akt and IP7 in diabetic rats during myocardial ischemia-reperfusion injury(MIRI). Methods A total of 32 SD male rats were randomly divided into a sham operation group, an ischemia reperfusion group, a renaulazine group and a renaulazine combined with Xueshuantong Capsule group. The heart rate, systolic blood pressure, diastolic blood pressure, left ventricular systolic blood pressure(LVSP), left ventricular diastolic blood pressure(LVDP), the maximum rate of increase of left ventricular pressure(+dp/dtmax), and the maximum rate of decrease of left ventricular pressure(-dp/dtmax));myocardial infarction area, autophagy level and myocardial IP7, p-NF-κB, p-AKT levels were observed. Results The LVSP, LVDP, +dp/dtmax and-dp/dtmax in the ischemia/reperfusion group, the ranolazine group and the lenolazine combined Xueshuantong group were significantly lower than those in the sham group(P<0.05). Compared with the ischemia reperfusion group, the LVSP, LVDP, +dp/dtmax and-dp/dtmax of the two groups were significantly increased, and lenolazine combined Xueshuantong group were significantly higher than the lenolazine group(P<0.05);there was no significant difference in myocardial ischemia between the three groups(P>0.05). Compared with the ischemia reperfusion group, the degree of myocardial infarction in the ranolazine group and the lenolazine combined Xueshuantong group was significantly decreased. The ranolazine combined with Xueshuantong group was significantly lower than the ranolazine group, the difference was statistically significant(P < 0.05);compared with the ischemia reperfusion group, beclin-1 protein and LC3-II protein expressions in ranolazine group and the lenolazine combined Xueshuantong group were significantly decreased in the two groups(P<0.05), and the improvement degree in ranolazine group was superior to that in the ischemia reperfusion group(P<0.05);compared with the ischemia repe

关 键 词：雷诺嗪 血栓通胶囊 肌缺血再灌注损伤 细胞自噬 

分 类 号：R541.4[医药卫生—心血管疾病] R972[医药卫生—内科学]