发光寿命响应型探针在时间分辨生物检测与成像中的应用研究  

Luminescence lifetime-responsive probes for time-resolved biosensing and bioimaging

作　　者：黄维[1,2,3,4] 吴琦[1,2,3] 赵强 HUANG Wei;WU Qi;ZHAO Qiang(Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,China;Institute of Flexible Electronics(Future Technology),Nanjing University of Posts and Telecommunications,Nanjing 210023,China;Key Laboratory for Organic Electronics and Information Displays,Nanjing University of Posts and Telecommunications,Nanjing 210023,China;Frontiers Science Center for Flexible Electronics(FSCFE),Northwestern Polytechnical University,Xi'an 710072,China)

机构地区：[1]南京邮电大学信息材料与纳米技术研究院,江苏南京210023 [2]南京邮电大学柔性电子(未来技术)研究院,江苏南京210023 [3]南京邮电大学有机电子与信息显示国家重点实验室培育基地,江苏南京210023 [4]西北工业大学柔性电子前沿科学中心,陕西西安710072

出　　处：《南京邮电大学学报（自然科学版）》2020年第5期27-42,共16页Journal of Nanjing University of Posts and Telecommunications：Natural Science Edition

基　　金：国家杰出青年科学基金(61825503);国家自然科学基金(21671108)资助项目。

摘　　要：近年来,发光寿命响应型探针在生物检测与成像领域的应用取得了显著进展。文中系统介绍了不同寿命响应型探针的设计及应用,包括有机染料、金属配合物、纳米粒子等。其中,长寿命探针可通过时间门控发光成像技术区别生物组织短寿命荧光信号,降低背景荧光干扰,提高检测信噪比。而不同衰减速率的发光信号则可以通过发光寿命成像技术在时域上区分,分析每一个像素的衰减信息,提高成像对比度和灵敏度。In recent years,the application of luminescence lifetime-responsive probes in the field of biolog­ical detection and imaging has made significant progress.This paper systematically introduces design strat­egies and applications of luminescence lifetime-responsive probes for bioimaging and biosensing,including organic dyes,transition metal complexes,and nanoparticles,etc.Among them,long-lived probes can be distinguished from short-lived autofluorescence of biological tissues by time-gated photoluminescence ima­ging,minimizing the influence of autofluorescence and improving the signal-to-noise ratio values.The sig­nals with different decay rates can be separated in the time domain by photoluminescence lifetime imaging and further report the decay details of each pixel,enriching the details of imaging and improving the ima­ging contrast and the sensitivity.

关 键 词：生物检测与成像 探针 时间分辨发光成像 时间门控发光成像 发光寿命成像 

分 类 号：Q-334[生物学] O657.3[理学—分析化学]