叶酸对大鼠肺缺血再灌注损伤的保护作用  

Protective effect of folic acid on lung ischemia-reperfusion injury in rats

作　　者：夏艳 马晓冬[2] 谢云斌 邵东华[2] XIA Yan;MA Xiao-dong;XIE Yun-bin;SHAO Dong-hua(Department of Anesthesiology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001;Department of Anesthesiology, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002;Department of Anesthesiology, Changzhou First People′s Hospital, Changzhou Jiangsu 213000, China)

机构地区：[1]江苏大学附属医院麻醉科,江苏镇江212001 [2]江苏大学附属人民医院麻醉科,江苏镇江212002 [3]常州市第一人民医院麻醉科,江苏常州213000

出　　处：《江苏大学学报（医学版）》2020年第6期510-514,共5页Journal of Jiangsu University：Medicine Edition

基　　金：镇江市社会发展项目(SH20160130127)。

摘　　要：目的:探讨叶酸对大鼠肺缺血再灌注损伤的影响。方法:选取健康成年雄性SD大鼠30只,随机均分为5组,每组6只,其中3组叶酸组分别以5、10、20 mg/d叶酸灌胃1周;然后叶酸组及缺血再灌注组大鼠构建肺缺血再灌注模型,夹闭左肺门30 min,再灌注2 h;假手术组游离左肺门不夹闭;HE染色观察每组大鼠肺组织病理变化;ELISA法测定大鼠血清IL-8和TNF-α水平。结果:与假手术组比较,缺血再灌注组大鼠肺组织病理损伤明显较重(P<0.01);与缺血再灌注组比较,叶酸组肺组织病理损伤明显较轻(P<0.01);与5 mg/d叶酸组比较,10 mg/d与20 mg/d叶酸组肺组织病理损伤明显较轻(P<0.01),而后两组比较未见显著性差异(P>0.05)。与假手术组比较,缺血再灌注组、叶酸组IL-8和TNF-α表达水平均明显增高(P<0.01);与缺血再灌注组比较,叶酸组IL-8和TNF-α表达水平明显降低(P<0.01);与5 mg/d叶酸组比较,10 mg/d与20 mg/d叶酸组TNF-α表达水平明显降低(P<0.05),而后两组比较差异无统计学意义(P>0.05);叶酸组IL-8水平降低呈一定的剂量依赖性(P<0.05)。结论:叶酸对大鼠肺缺血再灌注损伤有一定的保护作用,且在一定范围内随叶酸剂量的增加而增强。Objective:To explore the effect of folic acid on lung ischemia-reperfusion injury in rats.Methods:Thirty healthy adult male SD rats were randomly divided into 5 groups with 6 rats in each group,of which 3 groups were gavaged with folic acid at doses of 5,10,20 mg/d for 1 week.Then,the folic acid groups and ischemia-referfusion group rats were constructed a model of lung ischemia-reperfusion,in which the left hilum was clamped for 30 min and reperfused for 2 h;sham operation group the left hilus was dissociated but not clamped.The pathological changes of lung tissue was observed by HE staining;the level of IL-8 and TNF-αwere determined by ELISA.Results:Compared with the sham ope-ration group,the lung injury severity in the ischemia-reperfusion group was significantly serious(P<0.01);compared with the ischemia-reperfusion group,the severity in the three folic acid groups was significantly alleviated;compared with the 5 mg/d folic acid group,10 mg/d and 20 mg/d folic acid groups had significantly less pathological lesions in the lung tissue(P<0.01),while there was no significant difference between the later two groups(P>0.05).Compared with the sham operation group,the level of IL-8 and TNF-αin the ischemia-reperfusion group and the folic acid groups was remarkably higher(P<0.01);compared with the ischemia-reperfusion group,the level of IL-8 and TNF-αin the three folic acid groups was markedly lower(P<0.01);compared with the 5 mg/d folic acid group,the level of TNF-αin 10 mg/d and 20 mg/d folic acid groups was greatly lower;while there was no significant diffe-rence between the later two groups(P>0.05);the decrease of IL-8 level in the three folic acid groups was in a dose-dependent manner(P<0.05).Conclusion:Folic acid exerts protection on lung ischemia-reperfusion injury in rats.

关 键 词：叶酸 肺缺血再灌注损伤 IL-8 TNF-Α 大鼠 

分 类 号：R602[医药卫生—外科学] R392.11[医药卫生—临床医学]