双载药双重敏感壳聚糖接枝聚合物的制备及药物缓释  

Preparation and Sustained Drug Release of Chitosan-Grafted Polymer with Dual Drug Loading and Dual Sensitivity

作　　者：陈霞 牛世伟 张雪静 朱利民[1] CHEN Xia;NIU Shiwei;ZHANG Xuejing;ZHU Limin(College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《东华大学学报（自然科学版）》2020年第5期754-759,778,共7页Journal of Donghua University(Natural Science)

基　　金：上海市科委国际科技合作基金资助项目(16410723700);教育部生物医用纺织材料111引智专项资助项目(B07024)。

摘　　要：采用具有pH响应性和优良生物相容性的壳聚糖(CS)作为基底材料,通过可逆加成断裂链转移(reversible addition fragmentation chain transfer,RAFT)聚合法接枝温敏性材料N-乙烯基己内酰胺(NVCL),合成具有温度/pH双重敏感的聚合物CS-g-PNVCL。CS-g-PNVCL与阿霉素(DOX)和齐墩果酸(OA)通过物理作用,形成载药纳米颗粒(DOX/OA)@CS-g-PNVCL。采用傅里叶红外光谱仪和核磁共振氢谱对CS-g-PNVCL聚合物进行表征,采用扫描电子显微镜、透射电子显微镜以及动态光散射仪对(DOX/OA)@CS-g-PNVCL聚合物进行表征。结果表明,CS-g-PNVCL聚合物接枝成功,并且(DOX/OA)@CS-g-PNVCL形态分布良好。载药和体外释药结果显示(DOX/OA)@CS-g-PNVCL对温度和pH均有响应性,表明其具有良好的载药释药能力。This paper synthesized the polymer of CS-g-PNVCL with dual sensitivity of temperature and pH,taking chitosan(CS)as the substrate material and then grafting it onto N-vinyl caprolactam(NVCL)by the reversible addition fragmentation chain transfer(RAFT)polymerization.The chitosan has pH response and good biocompatibility,while the NVCL is sensitive to the temperature.The drug-loading nanoparticles of(DOX/OA)@CS-g-PNVCL were obtained through the physical action between CS-g-PNVCL and doxorubicin(DOX)or oleanolic acid(OA).The CS-g-PNVCL was characterized by Fourier transform infrared spectrometer and nuclear magnetic resonance spectroscopy.The(DOX/OA)@CS-g-PNVCL was characterized by scanning electron microscopy,transmission electron microscopy and dynamic light scattering.The results show that CS-g-PNVCL has been successfully synthesized and the(DOX/OA)@CS-g-PNVCL has a good morphological distribution.Besides,the(DOX/OA)@CS-g-PNVCL is responsive to both temperature and pH,indicating a good ability of drug loading and release in vitro experiments.

关 键 词：壳聚糖 可逆加成断裂链转移聚合 载药 体外释药 双重敏感 缓释 

分 类 号：O636.1[理学—高分子化学]