5℃条件下去精清驴精子不同保存时间对其活力和母驴受胎率的影响  

Effect of different storage time at 5℃cryopreservation on donkey sperm motility and pregnancy rate without seminal plasma

作　　者：刘冰 尹桂军 李敏 李雪贤 于杰 王涛 高鹏 徐传飞 李楠[2] 刘桂芹[3] 吴帅帅 LIU Bing;YIN Guijun;LI Min;LI Xuexian;YU Jie;WANG Tao;GAO Peng;XU Chuanfei;LI Nan;LIU Guiqin;WU Shuaishuai(Dong-E-E-Jiao Co.Ltd./National Engineering Research Center for Gelatin-based Traditional Chinese Medicine,Donge 252201,China;College of Animal Science and Technology,China Agriculture University,Beijing 100193,China;Agricultural College/Shandong Donkey Industry Technology Collaborative Innovation Center,Liaocheng University,Liaocheng 252059,China)

机构地区：[1]东阿阿胶股份有限公司/国家胶类中药工程技术研究中心,山东东阿252201 [2]中国农业大学动物科技学院,北京100193 [3]聊城大学农学院/山东省驴产业科技协同创新中心,山东聊城252059

出　　处：《黑龙江畜牧兽医》2020年第20期32-35,38,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目“经济动物高效安全养殖技术应用”子课题“驴冷冻冻精人工授精技术”(2018YFD0502205-2);山东省农业良种工程项目(2017LZGC020)。

摘　　要：为研究低温下去除精清的德州驴精子不同保存时间对其运动特性及母驴情期受胎率的影响,试验选择9头种公驴收集了50次射精的精液,去除精液中凝胶,离心后用INRA82稀释液重悬、稀释,获得精子密度为0.8×10^8个/mL的精液,取新鲜精液(0 h)及在5℃条件下保存24,48,72,96,120 h后的精液,使用计算机辅助精子活力分析仪检测精子的运动特性,并进行人工授精统计126头母驴情期受胎率。结果表明:与新鲜精液(0 h)相比,随着保存时间的延长,精子总活力、直线前进活力、平均运动速率及精子胞内ATP浓度逐渐降低。精液在5℃下保存时间不超过96 h时,母驴情期受胎率没有显著变化(P>0.05),而保存120 h的精液的情期受胎率显著低于0,24,48,72 h(P<0.05)。说明从精子中去除精清使驴精子免受5℃低温保存的损伤,且低温有效保存时间不超过72 h。To study the effects of different preservation time at low temperatures on sports performance and conception rate of dezhou donkey,50 ejaculations of semen from nine stud jackasses treated by removing gel were re-suspended and diluted with INRA82 diluent after centrifugation,subsequently,semen containing 0.8×10^8 sperm/mL was obtained.After 24 h,48 h,72 h,96 h or 120 h of cooled storage at 5℃,the motility characteristics of sperm and fresh sperm(0 h)were examined by a computer-aided sperm motility analyzer system,and the fetal rate of 126 female donkeys was calculated by artificial insemination.Obtained results showed that the total sperm motility,linear advancing motility,average movement rate and intracelluar ATP concentration in sperm in all groups decreased gradually with the extension of in vitro refrigeration time compared with fresh semen(0 h).When the semen was stored at 5℃for less than 96 h,there was no significant change in the pregnancy rate of the semen in the emotional stage(P>0.05),And the pregnancy rate of the semen stored for 120 h was significantly lower than that in the groups of 0 h,24 h,48 h,and 72 h(P<0.05).The results showed that semen without seminal plasma could protect donkey sperm from damage by 5℃cryopreservation,and the effective cryopreservation time was less than 72 h.

关 键 词：德州驴 去精清精子 低温 精子活力 人工授精 情期受胎率 

分 类 号：S822[农业科学—畜牧学]