不同产地罗汉松叶总黄酮提取工艺的优化与含量测定  

Optimization of extraction process and determination of total flavonoids in leaves of Podocarpus macrophyllus(Thunb.)D.Don from different origin

作　　者：刘睿 马家宝[2] 陆峥琳 魏江存 陈朝[2] 韦丽富 崔健[2] LIU Rui;MA Jia-bao;LU Zheng-lin;WEI Jiang-cun;CHEN Chao;WEI Li-fu;CUI Jian(Nanning Hospital of Traditional Chinese Medicine,Nanning 530001,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China;Guangxi International Zhuang Medicine Hospital,Nanning 530201,China)

机构地区：[1]南宁市中医医院,南宁530001 [2]广西中医药大学第一附属医院,南宁530023 [3]广西国际壮医医院,南宁530201

出　　处：《湖北农业科学》2020年第20期141-144,149,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(81360524);全国中药特色技术传承人才培训项目(T20194828003);2019年度广西高校中青年教师科研基础能力提升项目(2019KY0341);广西中医药大学青年基金项目(2019QN036);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室(国中医药发2009[21]号)。

摘　　要：采用回流提取法提取罗汉松[Podocarpus macrophyllus(Thunb.)D.Don]叶总黄酮,通过单因素试验,考察提取方法、提取溶剂体积分数、提取体积、提取时间对药材总黄酮含量的影响,采用正交试验优化提取工艺条件,筛选出罗汉松叶总黄酮的最佳提取工艺。结果表明,罗汉松叶总黄酮的最佳提取工艺为75%乙醇、料液比1∶30(g∶mL)、回流提取时间0.5 h。该方法重复性较好、稳定、可行。The reflux extraction method was used to extract total flavonoids from the leaves of Podocarpus macrophyllus(Thunb.)D.Don.The effects of extraction method,solvent concentration,extraction volume and extraction time on the content of total flavonoids in Podocarpus macrophyllus(Thunb.)D.Don were investigated by single factor test.The orthogonal test was used to optimize the extrac⁃tion conditions and the optimum extraction process was selected.The results showed that the optimum extraction process of total flavo⁃noids from the leaves of Podocarpus macrophyllus(Thunb.)D.Don was 75%ethanol,the ratio of material to liquid was 1∶30(g∶mL),and the reflux extraction time was 0.5 h.The method has good repeatability,stability and feasibility.

关 键 词：罗汉松[Podocarpus macrophyllus(Thunb.)D.Don] 叶 总黄酮 提取工艺 紫外可见-分光光度法 

分 类 号：R282[医药卫生—中药学]