响应面法优化ZTC1+1Ⅱ型澄清剂对三七茎叶皂苷提取液的除杂工艺  

Optimization for purifying technologyof the leaves and steams of Panax notoginseng saponins extract with ZTC1+1Ⅱ clarifier by response surface method

作　　者：秦枫[1,2] 李金贵[2] 朱善元[1] 王红昀 王安平[1] 吴双[1] QIN Feng;LI Jin-gui;ZHU Shan-yuan;WANG Hong-yun;WANG An-ping;WU Shuang(Jiangsu Agri-animal Husbandry Vocational College/Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Research,Taizhou 225300,Jiangsu,China;College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]江苏农牧科技职业学院/江苏省兽用生物制药高技术研究重点实验室,江苏泰州225300 [2]扬州大学兽医学院,江苏扬州225009

出　　处：《湖北农业科学》2020年第20期150-154,169,共6页Hubei Agricultural Sciences

基　　金：国家自然科学基金青年科学基金项目(31702286);江苏高校“青蓝工程”资助项目(00000217008);江苏农牧科技职业学院“凤凰人才工程”项目(10434014001)。

摘　　要：为确定ZTC1+1Ⅱ型天然澄清剂用于三七(Panax notoginseng)茎叶皂苷提取液除杂的最佳工艺,在单因素分析的基础上,以药物质量浓度、澄清剂用量、作用温度为自变量,以皂苷含量和固形物去除率作为评价指标,建立三因素三水平的Design-Expert响应面分析方法。结果表明,最佳除杂工艺为药液质量浓度(生药质量与皂苷提取液体积之比)为0.19 g/mL,ZTC1+1Ⅱ型天然澄清剂2组分加入顺序为先B组分后A组分,澄清剂B组分和A组分加入量分别为10%、5%药液体积,作用温度为40℃。在此工艺条件下进行三七茎叶皂苷提取液除杂工艺的优化,得出固形物去除率为28.40%,皂苷保留率为92.22%,其相对标准偏差(RSD)分别为1.74%和0.56%,重现性较好,验证了该模型的有效性。In order to determine the ZTC1+1Ⅱ type natural clarifying agent used for notoginseng(Panax notoginseng)leaves and steams glycosides extract the best technology of removing impurity,on the basis of single factor analysis,the concentration of drug quality,clarifying agent dosage,temperature is the independent variable,saponin content and solids removal rate as evaluation index,establishing three factors and three levels of Design-Expert response surface analysis method. The results show that the best process of Panax notoginseng leaves and steamspurificationis as follows:The mass concentration of liquid is 0.19 g/mL,the order of ZTC1+1Ⅱ natural clarifier is first part A and after part B,the dosage of clarifiers B and A are 10% and 5% volume of the extract,respectively,and acting temperature is 40 ℃. Under this technological condition,the removal process of stem and leaf of Panax notoginseng is optimized,and the solid removal rate is 28.40%,the saponin retention rate is 92.22%,and the RSD is 1.74% and 0.56%,respectively.The repeatability is good,which verified the validity of the model.

关 键 词：三七(Panax notoginseng)茎叶 ZTC1+1Ⅱ型澄清剂 皂苷含量 固形物去除率 响应面法 

分 类 号：S853[农业科学—临床兽医学]