原发性干燥综合征患者唇腺组织水通道蛋白5的表达及意义  

Expression of aquaporin 5 in labial gland of patients with primary Sjögren's syndrome and its significance

作　　者：王领[1,2] 张娜 王艳玲[1] 赵福涛[1] WANG Ling;ZHANG Na;WANG Yan-ling;ZHAO Fu-tao(Department of Rheumatology,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201999,China;Department of Rheumatology,Gongli Hospital,Shanghai Pudong New Area,Shanghai 200135,China)

机构地区：[1]上海交通大学医学院附属第九人民医院风湿免疫科,上海201999 [2]上海市浦东新区公利医院风湿免疫科,上海200135

出　　处：《上海交通大学学报（医学版）》2020年第10期1425-1429,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海交通大学医学院附属第三人民医院自然基金(syz2015-017)。

摘　　要：目的·探讨原发性干燥综合征(primary Sjögren's syndrome,pSS)患者唇腺组织中水通道蛋白5(aquaporin 5,AQP5)、血清白细胞介素-21(interleukin-21,IL-21)、β2微球蛋白(β2-microglobulin,β2-MG)的表达水平与病情活动之间的关系。方法·收集39例pSS患者的病例资料,检测红细胞沉降率(erythrocyte sedimentation rate,ESR)和免疫球蛋白G(immunoglobulin,IgG)等炎症指标。行唇腺活检,根据Chisholm唇腺病理进行分级,将所有标本分为Ⅰ、Ⅱ、Ⅲ、Ⅳ4组。免疫组织化学法检测唇腺组织AQP5的表达,ELISA法检测血清IL-21、β2-MG水平。结果·随着唇腺组织淋巴细胞数目的增加,AQP5蛋白表达量减少。唇腺组织AQP5的表达与IL-21、β2-MG、ESR、IgG呈负相关(r=-0.924,P=0.000;r=-0.366,P=0.022;r=-0.611,P=0.000;r=-0.741,P=0.000)。随着唇腺组织淋巴细胞数目的增加,血清IL-21表达增加,4组间IL-21的表达差异有统计学意义。血清β2-MG在各组间未见明显差异。结论·pSS患者唇腺组织AQP5的表达与淋巴细胞浸润有关,且AQP5、IL-21、β2-MG与病情活动度存在相关性,可作为生物学标志物,用于临床病情的评估。Objective·To investigate the expression level of aquaporin 5(AQP5)in labial gland,and interleukin-21(IL-21)andβ2-microglobulin(β2-MG)in serum of patients with primary Sjögren's syndrome(pSS)and their association with the activity of the disease.Methods·Thirty-nine patients with pSS were collected and inflammatory indicators such as erythrocyte sedimentation rate(ESR)and IgG were detected.Lip gland biopsy was performed.All specimens were divided into four groups according to Chisholm lip gland pathological classification:Ⅰ,Ⅱ,ⅢandⅣ.The expression of AQP5 in labial gland was detected by immunohistochemistry and the levels of IL-21 andβ2-MG in serum were detected by ELISA.Results·As the number of lymphocytes in labial gland increased,the expression of AQP5 protein decreased.The expression of AQP5 in labial gland was negatively correlated with IL-21,β2-MG,ESR and IgG(r=-0.924,P=0.000;r=-0.366,P=0.022;r=-0.611,P=0.000;r=-0.741,P=0.000).As the number of lymphocytes in labial gland increased,the expression of IL-21 in serum increased,and there was significant difference among four grades.There was no significant difference in serumβ2-MG among the groups.Conclusion·The expression of AQP5 in labial gland of patients with pSS is related to lymphocyte infiltration,and AQP5,IL-21 andβ2-MG are related to disease activity,which can be used as biological markers for clinical disease evaluation.

关 键 词：原发性干燥综合征 水通道蛋白5 白细胞介素-21 Β2微球蛋白 

分 类 号：R593.2[医药卫生—内科学]