高效液相色谱法测定纺织品中2种CMR物质  

Determination of Two CMRs in Textiles by High Performance Liquid Chromatography

作　　者：秦鑫[1] 徐韵扬 吴怡 顾虎[1] 胡喆[1] 王敏超 QINXin;XU Yun-yang;WU Yi;GU Hu;HUZhe;WANG Min-chao(Hangzhou Institute of Test and Calibration for Quality and Technology Supervision,Hangzhou 310019,China)

机构地区：[1]杭州市质量技术监督检测院,浙江杭州310019

出　　处：《纺织科技进展》2020年第11期5-8,共4页Progress in Textile Science & Technology

基　　金：国家重点研发项目(2017YFF0209600);浙江省质量技术监督系统科研项目(20190119);中国标准化研究院国家标准馆项目(JXJY-2017-266)。

摘　　要：建立了超声萃取结合高效液相色谱-荧光检测器(HPLC-FLD)同时测定纺织品中苯并(b)荧蒽和苯并(a)芘2种CMR物质的分析方法。样品采用甲醇超声萃取,经过滤后采用Agilent SB-C18色谱柱(100mm×4.6mm,3.5μm)对萃取液中目标物进行分离,通过荧光检测器(λex/λem=300nm/430nm)检测,色谱保留时间定性,外标法定量。试验结果表明,2种CMR物质在10~200μg/L范围内呈良好的线性关系,相关系数均大于0.999;方法定量限(S/N=10)0.1mg/kg;添加水平为0.2~1.0mg/kg时,测得回收率为89.6%~102.3%,相对标准偏差(RSD,n=6)为1.5%~3.2%。该方法具有前处理简便、回收率高、精密度好等优点,适用于测定纺织品中苯并(b)荧蒽和苯并(a)芘2种CMR物质。A novel method was established to determine two CMRs in textiles by high performance liquid chromatography-fluorescence detection combined with ultrasound extraction(UA-HPLC-FLD).Methanol was used as the extraction agent.Agilent SB-C18 chromatographic column(100 mm×4.6 mm,3.5μm)was adopted to separate the analytes.The analytes was detected by fluorescence detector(λex/λem=300/430 nm),qualified by chromatographic retention time,and quantified by the external standard method.Experimental results indicated that the two CMRs with content range of 10 to 200μg/L was better and correlation coefficients were larger than 0.999.The method limits of quantification(MLOQ)was 0.1 mg/kg(S/N=10).When the addition level was from 0.2 to 1.0 mg/kg,the recovery rate was 89.6%to 102.3%,the relative standard deviation was from 1.5%to 3.2%.It was considered that preprocessing of the method was convenient,and recovery rate was higher and precise,which was suitable to measure the CMRs in textiles.

关 键 词：CMR 高效液相色谱法 超声萃取 纺织品 

分 类 号：TS107[轻工技术与工程—纺织工程] O657.72[轻工技术与工程—纺织科学与工程]